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Dmrt1 对于中华鳖 Pelodiscus sinensis 的初级雄性性分化是必需的。

Dmrt1 is required for primary male sexual differentiation in Chinese soft-shelled turtle Pelodiscus sinensis.

机构信息

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, China.

Trinity School of Arts and Sciences, Duke University, Durham, NC, 27708, USA.

出版信息

Sci Rep. 2017 Jun 30;7(1):4433. doi: 10.1038/s41598-017-04938-5.

DOI:10.1038/s41598-017-04938-5
PMID:28667307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5493664/
Abstract

In vertebrates, the primary sex-determining signals that initiate sexual development are remarkably diverse, ranging from complete genetic to environmental cues. However, no sex determination-related genes have been functionally identified in reptiles. Here, we characterized a conserved DM domain gene, Dmrt1, in Chinese soft-shelled turtle Pelodiscus sinensis (P. sinensis), which exhibits ZZ/ZW sex chromosomes. Dmrt1 exhibited early male-specific embryonic expression, preceding the onset of gonadal sex differentiation. The expression of Dmrt1 was induced in ZW embryonic gonads that were masculinized by aromatase inhibitor treatment. Dmrt1 knockdown in ZZ embryos by RNA interference resulted in male to female sex reversal, characterized by obvious feminization of gonads, significant down-regulation of testicular markers Amh and Sox9, and remarkable up-regulation of ovarian regulators, Cyp19a1 and Foxl2. Conversely, ectopic expression of Dmrt1 led to largely masculinized genetic females, production of Amh and Sox9, and a decline in Cyp19a1 and Foxl2. These findings demonstrate that Dmrt1 is both necessary and sufficient to initiate testicular development, thereby acting as an upstream regulator of the male pathway in P. sinensis.

摘要

在脊椎动物中，启动性发育的主要性别决定信号非常多样化，从完全的遗传信号到环境信号都有涉及。然而，在爬行动物中尚未鉴定到与性别决定相关的基因。在这里，我们对具有 ZZ/ZW 性染色体的中华鳖（Pelodiscus sinensis）中的一个保守的 DM 结构域基因 Dmrt1 进行了研究。Dmrt1 在雄性胚胎中具有早期的特异性表达，早于性腺性别分化的开始。用芳香酶抑制剂处理雄性化的 ZW 胚胎性腺可诱导 Dmrt1 的表达。通过 RNA 干扰使 ZZ 胚胎中的 Dmrt1 敲低会导致雄性到雌性的性反转，表现为性腺明显的雌性化，睾丸标志物 Amh 和 Sox9 的显著下调，以及卵巢调控因子 Cyp19a1 和 Foxl2 的显著上调。相反，Dmrt1 的异位表达导致遗传上的雌性个体大部分雄性化，产生 Amh 和 Sox9，并下调 Cyp19a1 和 Foxl2。这些发现表明 Dmrt1 既是睾丸发育所必需的，也是启动的充分条件，因此它在中华鳖中是雄性途径的上游调控因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/ed4c2f51ec97/41598_2017_4938_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/8951afe5809b/41598_2017_4938_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/51981d409dbd/41598_2017_4938_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/3f35901f347b/41598_2017_4938_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/a01b77db134e/41598_2017_4938_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/6057807ebe21/41598_2017_4938_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/fade6e575cd4/41598_2017_4938_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/ed4c2f51ec97/41598_2017_4938_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/8951afe5809b/41598_2017_4938_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/51981d409dbd/41598_2017_4938_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/3f35901f347b/41598_2017_4938_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/a01b77db134e/41598_2017_4938_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/6057807ebe21/41598_2017_4938_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/fade6e575cd4/41598_2017_4938_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ba/5493664/ed4c2f51ec97/41598_2017_4938_Fig7_HTML.jpg

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