识别循环微泡上氧化特异性表位的天然IgM抗体的特性分析

Characterization of Natural IgM Antibodies Recognizing Oxidation-Specific Epitopes on Circulating Microvesicles.

作者信息

Puhm Florian, Binder Christoph J

机构信息

Department of Laboratory Medicine, Medical University of Vienna, Lazarettgasse 14, AKH BT 25.2/6, 1090, Vienna, Austria.

Research Center for Molecular Medicine (CeMM) of the Austrian Academy of Sciences, 1090, Vienna, Austria.

出版信息

Methods Mol Biol. 2017;1643:147-154. doi: 10.1007/978-1-4939-7180-0_11.

DOI:10.1007/978-1-4939-7180-0_11

PMID:28667535

Abstract

Natural IgM antibodies specific for oxidation-specific epitopes (OSEs) have been found to bind to circulating microvesicles (MVs), also known as microparticles. This chapter describes how endogenous natural IgM antibodies bound to circulating MV subsets can be characterized on the one hand, and how monoclonal natural IgM antibodies can be used to characterize subsets of circulating MVs on the other hand.

摘要

已发现对氧化特异性表位（OSEs）具有特异性的天然IgM抗体可与循环微泡（MVs）结合，循环微泡也称为微粒。本章一方面描述了如何对与循环MV亚群结合的内源性天然IgM抗体进行表征，另一方面描述了如何使用单克隆天然IgM抗体对循环MV亚群进行表征。