采用高效阴离子交换色谱-脉冲安培检测法（HPAEC-PAD）对低乳糖乳制品中的乳糖进行选择性和灵敏测定。
Selective and sensitive determination of lactose in low-lactose dairy products with HPAEC-PAD.
作者信息
van Scheppingen Wibo B, van Hilten Piet H, Vijverberg Marieke P, Duchateau Alexander L L
机构信息
DSM Biotechnology Center, P.O. Box 1, 2600 MA, Delft, The Netherlands.
DSM Biotechnology Center, P.O. Box 1, 2600 MA, Delft, The Netherlands.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Aug 15;1060:395-399. doi: 10.1016/j.jchromb.2017.06.024. Epub 2017 Jun 21.
The demand for low lactose dairy products is increasing and more different lactose free food is commercially available. The level of lactose in these products decreased during the last years and nowadays a concentration of <0.01% is generally accepted as "lactose free". For the determination of the lactose concentrations in these dairy products a sensitive analysis method is needed. We developed a method for the determination of low concentrations of lactose in a wide range of dairy products. A simple sample preparation with dilution, centrifugation and ultrafiltration is efficient for the isolation of lactose from the sample matrix. In this paper, a new HPAEC-PAD analysis on a CarboPac PA100 column gives a good separation of lactose from the other saccharides. This separation in combination with the PAD detector yields a selective and sensitive method for the quantification at the desired concentrations of lactose in low lactose dairy products.
对低乳糖乳制品的需求正在增加，市场上有更多种类的无乳糖食品可供选择。在过去几年中，这些产品中的乳糖含量有所下降，如今，乳糖浓度<0.01%通常被认为是“无乳糖”。为了测定这些乳制品中的乳糖浓度，需要一种灵敏的分析方法。我们开发了一种测定多种乳制品中低浓度乳糖的方法。通过稀释、离心和超滤进行简单的样品制备，可有效地从样品基质中分离出乳糖。本文采用新型高效阴离子交换色谱-脉冲安培检测法（HPAEC-PAD），在CarboPac PA100柱上实现了乳糖与其他糖类的良好分离。这种分离方法与脉冲安培检测器相结合，为低乳糖乳制品中所需浓度的乳糖定量提供了一种选择性强且灵敏的方法。
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