Sanders Peter, Ernste-Nota Veronica, Visser Klaas, van Soest Jeroen, Brunt Kommer
Eurofins Carbohydrates Competence Centre, PO Box 766, 8440 AT Heerenveen, The Netherlands.
Rotating Disc BV, Spoorlaan 31, 9753 HV Haren, The Netherlands.
J AOAC Int. 2017 Sep 1;100(5):1577-1581. doi: 10.5740/jaoacint.17-0068. Epub 2017 May 11.
A method using high-performance anion-exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD) for the determination of mono- and disaccharides is described. The method was accepted by the International Dairy Federation and the Internal Organization for Standardization as a new work item for the determination of sugars in dairy matrixes, and the Milk and Milk Products technical committee of ISO/TC 34/SC 5 accepted the topic "Milk and milk products - Determination of the sugar contents - High-performance anion-exchange chromatographic method (HPAEC-PAD)" as a new work item. The proposed method consists of an aqueous ethanol extraction of the sugars in the dairy sample, followed by clarification with Carrez I and II reagents. The clarified filtrate is diluted and then directly introduced in the HPAEC-PAD system for quantification of the sugars. A single-laboratory validation of the proposed method has been scheduled for spring 2017.
本文描述了一种使用带脉冲安培检测器（PAD）的高效阴离子交换色谱法（HPAEC）测定单糖和双糖的方法。该方法被国际乳品联合会和国际标准化组织接受为乳制品基质中糖类测定的新工作项目，并且ISO/TC 34/SC 5的乳与乳制品技术委员会接受了“乳与乳制品 - 糖类含量的测定 - 高效阴离子交换色谱法（HPAEC-PAD）”这一主题作为新工作项目。所提出的方法包括用乙醇水溶液提取乳制品样品中的糖类，然后用Carrez I和II试剂进行澄清。澄清后的滤液经稀释后直接引入HPAEC-PAD系统进行糖类定量。已计划于2017年春季对所提出的方法进行单实验室验证。
