Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, PR China.
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, PR China.
Water Res. 2017 Oct 15;123:206-215. doi: 10.1016/j.watres.2017.06.066. Epub 2017 Jun 23.
Recently, the influence of metal oxide nanoparticles (NPs) on methanogenesis in anaerobic digestion has drawn much attention, however, the changes in NPs and functioning consortia within the methanogenic community are usually not investigated. Therefore, the methanogenesis performance, NPs transformation and methanogenic community development in anaerobic digester sludge under MnO NP supplementation were demonstrated in this study. MnO NPs (400 mg/gVSS) stimulated the methane (CH) yield by 42% for a final CH proportion of 81.8% of the total gas production. Meanwhile, the coenzyme F and INT-electron transport system activities showed positive correlation with MnO concentration. Microbial Mn reduction and oxidation occurred in conjunction with methanogenesis, resulting in transformation of the shape of the MnO NPs from wire-like to globular particles. Microbial community analysis indicated that the relative abundances of genera Methanobacterium, Methanosaeta, and Methanosarcina were higher in the presence of MnO NPs. Moreover, a new and different crucial synergy within the methanogenic community was formed with low-abundance consortia driving Mn respiration coupled to methanogenesis in anaerobic digestion. To our knowledge, this is the first report on transformation of metal oxides NPs combined with syntrophic community development in studies focusing on methanogenesis in response to NPs.
最近，金属氧化物纳米颗粒（NPs）对厌氧消化中产甲烷的影响引起了广泛关注，然而，通常不会研究 NPs 的变化和产甲烷菌群落中的功能群落。因此，本研究中在 MnO NP 补充下，研究了厌氧消化污泥中产甲烷性能、NPs 转化和产甲烷菌群落的发展。MnO NPs（400mg/gVSS）刺激甲烷（CH）产量增加了 42%，最终 CH 占总产气量的 81.8%。同时，辅酶 F 和 INT-电子传递系统活性与 MnO 浓度呈正相关。微生物 Mn 还原和氧化与产甲烷作用同时发生，导致 MnO NPs 的形状从线状变为球形颗粒。微生物群落分析表明，在存在 MnO NPs 的情况下，Methanobacterium、Methanosaeta 和 Methanosarcina 的相对丰度更高。此外，在厌氧消化中产甲烷过程中，形成了一个新的、不同的关键协同作用，低丰度群落驱动 Mn 呼吸与产甲烷作用偶联。据我们所知，这是第一篇关于金属氧化物 NPs 转化以及针对 NPs 作用下产甲烷反应中协同群落发展的研究报告。
