源自[未提及具体来源]的脂多糖可促进小鼠小胶质细胞中β淀粉样蛋白的吞噬活性。
Lipopolysaccharides Derived from Can Promote the Phagocytic Activity of Amyloid β in Mouse Microglial Cells.
作者信息
Kobayashi Yutaro, Inagawa Hiroyuki, Kohchi Chie, Okazaki Katsuichiro, Zhang Ran, Kobara Hideki, Masaki Tsutomu, Soma Gen-Ichiro
机构信息
Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
出版信息
Anticancer Res. 2017 Jul;37(7):3917-3920. doi: 10.21873/anticanres.11774.
BACKGROUND/AIM: Recent studies reported that lipopolysaccharide (LPS) exhibits beneficial effects on prevention of immune-related diseases by activating macrophages. We previously demonstrated that pre-treatment with LPS derived from Pantoea agglomerans (LPSp) activated amyloid β (Aβ) phagocytosis in mouse primary microglia. In the present study, we further examined the promotory effect on phagocytosis of phagocytic particles in the C8-B4 microglia cell line.
MATERIALS AND METHODS
Phagocytic analysis of C8-B4 cells was evaluated using phagocytic particles (latex beads or HiLyte™ Fluor 488-conjugated Aβ).
RESULTS
The phagocytic activity of latex beads was dependent on the concentration of beads and incubation time. LPSp, at as low as 100 pg/ml, significantly increased phagocytosis against the beads. In the experiment of Aβ phagocytosis, LPSp significantly increased Aβ phagocytic activity.
CONCLUSION
LPSp treatment was confirmed to enhance Aβ phagocytosis by mouse microglia. It is suggested that the use of LPSp may be a potential promising candidate for the prevention of Alzheimer's disease.
背景/目的：最近的研究报道，脂多糖（LPS）通过激活巨噬细胞对预防免疫相关疾病具有有益作用。我们之前证明，用成团泛菌来源的LPS（LPSp）预处理可激活小鼠原代小胶质细胞中的淀粉样β蛋白（Aβ）吞噬作用。在本研究中，我们进一步研究了其对C8-B4小胶质细胞系中吞噬颗粒吞噬作用的促进效果。
材料与方法
使用吞噬颗粒（乳胶珠或HiLyte™ Fluor 488标记的Aβ）评估C8-B4细胞的吞噬分析。
结果
乳胶珠的吞噬活性取决于珠子的浓度和孵育时间。低至100 pg/ml的LPSp显著增加了对珠子的吞噬作用。在Aβ吞噬实验中，LPSp显著增加了Aβ吞噬活性。
结论
证实LPSp处理可增强小鼠小胶质细胞的Aβ吞噬作用。提示使用LPSp可能是预防阿尔茨海默病的一个潜在有前景的候选方法。
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