用高效液相色谱-原子吸收分光光度法分离和表征两种海豹体内的金属硫蛋白。

Separation and characterization of metallothionein in two species of seals by high performance liquid chromatography-atomic absorption spectrophotometry.

作者信息

Mochizuki Y, Suzuki K T, Sunaga H, Kobayashi T, Doi R

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;82(2):249-54. doi: 10.1016/0742-8413(85)90158-6.

Abstract

Metallothionein (MT) was separated from the livers of two species of seals, ribbon seal (Histriophoca fasciata) and harbour seal (Phoca vitulina), by high performance liquid chromatography--atomic absorption spectrophotometry (HPLC-AAS). Both gel permeation and anion-exchange HPLC demonstrated that MT was present as a mixture of at least two isoforms in the livers of both species of seals.

摘要

通过高效液相色谱 - 原子吸收光谱法（HPLC - AAS）从两种海豹，即带纹海豹（Histriophoca fasciata）和港海豹（Phoca vitulina）的肝脏中分离出金属硫蛋白（MT）。凝胶渗透和阴离子交换高效液相色谱均表明，在这两种海豹的肝脏中，MT均以至少两种同工型的混合物形式存在。