Yousri R, Hanke W
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;82(2):283-90. doi: 10.1016/0742-8413(85)90164-1.
Liver tissue of carp was kept in roller tubes and the basal and epinephrine-induced release of glucose after 6 or 24 hr incubation were measured. The amount of liver glycogen after incubation was also determined. The liver was taken from carp treated in vivo with pollutants, mainly PCP or phenol, or was exposed to these pollutants in vitro. Treatment of carp in vivo with 10-10(4) micrograms/l phenol reduced the basal and the epinephrine-induced release of glucose from the liver. Treatment with low doses increased the glycogen content of the liver slightly, treatment with higher doses reduced the amount. Treatment of carp with low doses of several pollutants decreased mainly the basal glucose release from the liver and reduced the glycogen content. In vitro incubation of the liver with PCP or phenol for 3 days reduced at first the basal release and later the epinephrine-stimulated release of glucose from the liver. After a few days the glycogen content of liver exposed to pollutants was more strongly reduced than that of controls. The phosphorylase activity was slightly increased in liver tissue by the pollutants.
鲤鱼肝脏组织保存在滚瓶中，测定孵育6小时或24小时后基础状态下以及肾上腺素诱导的葡萄糖释放量。同时也测定孵育后肝脏糖原的含量。肝脏取自体内用污染物（主要是五氯苯酚或苯酚）处理过的鲤鱼，或者取自体外暴露于这些污染物的鲤鱼。体内用10 - 10⁴微克/升苯酚处理鲤鱼，会降低肝脏基础状态下以及肾上腺素诱导的葡萄糖释放。低剂量处理会使肝脏糖原含量略有增加，高剂量处理则会使其降低。用低剂量的几种污染物处理鲤鱼，主要会降低肝脏基础葡萄糖释放量并减少糖原含量。肝脏在体外与五氯苯酚或苯酚孵育3天，起初会降低基础葡萄糖释放，随后会降低肾上腺素刺激的葡萄糖释放。几天后，暴露于污染物的肝脏糖原含量比对照组降低得更明显。污染物会使肝脏组织中的磷酸化酶活性略有增加。
