Dierickx P J
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;82(2):495-500. doi: 10.1016/0742-8413(85)90199-9.
Glutathione S-transferase in the cytosol of rainbow trout liver was partially purified by affinity chromatography on a column with glutathione coupled to epoxy-activated Sepharose 6B, which retained 94% of the total activity. Chromatofocussing on a Polybuffer exchanger 118 column separated the glutathione S-transferase into six major cationic isoenzymes (K1-K6), and some minor fractions. SDS-polyacrylamide slab gel electrophoresis showed K1-K3 to be heterodimers with subunits of Mr 25,000 and 26,500, and K4-K6 to be homodimers with subunits of Mr 25,000. The glutathione S-transferase isoenzymes were partially characterized by different biochemical parameters. The hepatic rainbow trout glutathione S-transferases were inhibited by the organic water pollutants, 1,4-benzoquinone and 2,4-dichlorophenoxyacetic acid. The same kinetic inhibition patterns were observed with these inhibitors as for rat liver glutathione S-transferases. It is concluded that rainbow trout glutathione S-transferases can play a key role in the detoxication of organic micropollutants in the aquatic environment.
通过在与谷胱甘肽偶联到环氧活化的琼脂糖凝胶6B上的柱子上进行亲和层析，对虹鳟鱼肝细胞质中的谷胱甘肽S-转移酶进行了部分纯化，该柱子保留了94%的总活性。在聚缓冲液交换剂118柱上进行色谱聚焦，将谷胱甘肽S-转移酶分离为六种主要的阳离子同工酶（K1-K6）和一些次要组分。SDS-聚丙烯酰胺平板凝胶电泳显示K1-K3是由分子量为25,000和26,500的亚基组成的异二聚体，K4-K6是由分子量为25,000的亚基组成的同二聚体。通过不同的生化参数对谷胱甘肽S-转移酶同工酶进行了部分表征。肝虹鳟谷胱甘肽S-转移酶受到有机水污染物1,4-苯醌和2,4-二氯苯氧乙酸的抑制。观察到这些抑制剂对虹鳟谷胱甘肽S-转移酶的动力学抑制模式与对大鼠肝谷胱甘肽S-转移酶的相同。得出的结论是，虹鳟谷胱甘肽S-转移酶在水生环境中有机微污染物的解毒过程中可以发挥关键作用。
