State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Virus Res. 2017 Jun 15;238:179-182. doi: 10.1016/j.virusres.2017.06.017. Epub 2017 Jun 30.
In this study, we report the identification and characterization of a highly evolved type 3 vaccine-derived poliovirus (VDPV) strain designated as WIV14, isolated in 2014 from a 4-year-old child suspected of having an enteroviral infection in China. Complete genome sequence of WIV14 revealed multiple nucleotide substitutions when compared with the attenuated poliovirus (PV) Sabin 3, including the reversion of three major attenuation sites to wild type. From the nucleotide divergence for the P1/capsid region, we estimated that the evolution time of WIV14 was more than 7 years, indicating the possible long time of replication. WIV14 strain seemed to have differences in biological characteristics compared with attenuated PV strains, such as being non-temperature-sensitive and producing large plaques. The current isolation of a highly divergent type 3 VDPV gives an idea of the risk of emergent VDPV strains, and emphasizes the importance of maintaining high vaccination coverage and herd immunity against PVs in China.
在这项研究中，我们报告了一种高度进化的 3 型疫苗衍生脊髓灰质炎病毒（VDPV）株的鉴定和特征，该病毒株被命名为 WIV14，于 2014 年在中国从一名 4 岁疑似肠道病毒感染的儿童中分离得到。WIV14 的完整基因组序列与减毒脊髓灰质炎病毒（PV）Sabin 3 相比，发现了多个核苷酸取代，包括将三个主要的衰减位点恢复为野生型。从 P1/衣壳区域的核苷酸差异来看，我们估计 WIV14 的进化时间超过 7 年，表明其可能有长时间的复制。与减毒 PV 株相比，WIV14 株在生物学特性上似乎存在差异，例如非温度敏感和产生大的噬菌斑。目前分离出的高度分化的 3 型 VDPV 表明了新出现的 VDPV 株的风险，强调了在中国维持高疫苗接种覆盖率和群体免疫以预防 PV 的重要性。
