Tremblay J, Gerzer R, Pang S C, Cantin M, Genest J, Hamet P
FEBS Lett. 1986 Jan 6;194(2):210-4. doi: 10.1016/0014-5793(86)80086-2.
Particulate guanylate cyclase from bovine adrenal cortex can be stimulated by ANF. A 2-fold stimulation of the enzyme was obtained with 100 nM ANF and a half-maximal stimulation, with a 5 nM dose. The stimulation by ANF persisted for at least 30 min. Various detergents, such as Triton X-100, Lubrol PX, cholate, CHAPS, digitonin and zwittergent, stimulated several-fold the activity of particulate guanylate cyclase. However, only Triton X-100 dispersed particulate guanylate cyclase without affecting its response to ANF. The dose-response curve of ANF stimulation of the particulate and the Triton X-100 dispersed enzyme was similar. The dispersion of a fully responsive guanylate cyclase to ANF will help us to uncover the type of interactions between guanylate cyclase and ANF. It will also be used as a first step for the purification of an ANF-sensitive particulate guanylate cyclase.
来自牛肾上腺皮质的颗粒型鸟苷酸环化酶可被心房钠尿肽（ANF）激活。100 nM的ANF可使该酶的活性提高2倍，5 nM的剂量可产生半数最大激活效应。ANF的激活作用至少持续30分钟。多种去污剂，如Triton X-100、Lubrol PX、胆酸盐、CHAPS、洋地黄皂苷和两性离子去污剂，可使颗粒型鸟苷酸环化酶的活性提高数倍。然而，只有Triton X-100能分散颗粒型鸟苷酸环化酶，且不影响其对ANF的反应。颗粒型鸟苷酸环化酶以及经Triton X-100分散后的酶对ANF刺激的剂量反应曲线相似。将对ANF完全有反应的鸟苷酸环化酶分散，将有助于我们揭示鸟苷酸环化酶与ANF之间的相互作用类型。这也将作为纯化对ANF敏感的颗粒型鸟苷酸环化酶的第一步。
