[化香树提取物诱导人鼻咽癌CNE1和CNE2细胞发生类凋亡的机制]
[Mechanism of Platycarya strobilacea Sieb. et Zucc extract-induced methuosis in human nasopharyngeal carcinoma CNE1 and CNE2 cells].
作者信息
Zhu Jun-Yu, Tu Wei, Zeng Chao, Mao Heng-Xu, DU Qing-Feng, Cai Hong-Bing
机构信息
1Southern Medical University, Guangzhou 510515, China; Guangzhou 510315, China; 3Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Jun 20;37(6):827-832. doi: 10.3969/j.issn.1673-4254.2017.06.20.
OBJECTIVE
To study the effect of Platycarya strobilacea Sieb. et Zucc (PSZ) extract on methuosis of human nasopharyngeal carcinoma CNE1 and CNE2 cells and explore the underlying mechanism.
METHODS
CNE1 and CNE2 cells were treated with 1 mg/mL PSZ extract and the expressions of Rac1 mRNA and Rac1 protein were detected using RT-qPCR and Western blotting, respectively. Results CNE1 and CNE2 cells showed obvious morphological changes typical of methuosis following treatment with PSZ extract characterized by cell merging, accumulation of large cytoplasmic vacuoles, and membrane rupture without obvious changes in the nuclei. PSZ treatment resulted in up-regulated Rac1 mRNA and Rac1 protein expressions in the cells. Application of EHT 1864 obviously blocked the effect of PSZ extract in inducing methuosis in CNE1 and CNE2 cells.
CONCLUSION
PSZ extract can induce methuosis in CNE1 and CNE2 cells by inducing the overexpression of Rac1.
目的
研究化香树果序提取物对人鼻咽癌CNE1和CNE2细胞自噬性死亡的影响，并探讨其潜在机制。
方法
用1mg/mL化香树果序提取物处理CNE1和CNE2细胞，分别采用RT-qPCR和蛋白质免疫印迹法检测Rac1 mRNA和Rac1蛋白的表达。结果化香树果序提取物处理后的CNE1和CNE2细胞呈现出自噬性死亡典型的明显形态学变化，特征为细胞融合、大量细胞质空泡积聚和细胞膜破裂，而细胞核无明显变化。化香树果序处理使细胞中Rac1 mRNA和Rac1蛋白表达上调。应用EHT 1864明显阻断了化香树果序提取物诱导CNE1和CNE2细胞自噬性死亡的作用。
结论
化香树果序提取物可通过诱导Rac1过表达诱导CNE1和CNE2细胞自噬性死亡。
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