Giachero María L, Marquez Nathalie, Gallou Adrien, Luna Celina M, Declerck Stéphane, Ducasse Daniel A
Instituto de Patología Vegetal - Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología AgropecuariaCórdoba, Argentina.
Consejo Nacional de Investigaciones Científicas y TécnicasCórdoba, Argentina.
Front Plant Sci. 2017 Jun 16;8:1033. doi: 10.3389/fpls.2017.01033. eCollection 2017.
In this work, we described an system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/ Pre-mycorrhized plantlets with were infected by either locally via a plug of gel supporting mycelium (Method 1) or via a macroconidia suspension applied to the medium surface (Method 2). Root colonization by the AMF and infection by the pathogen were similar to the usual observations in pot experiments. Within a period of 18 days, more than 20% of the roots were colonized by the AMF and infection by the pathogen was observed in all the plants. In presence of AMF, a decrease in symptoms and in the level of root tissue infection was noticed. With Method 1, smaller necrotic lesions were observed in the pre-mycorrhized plantlets. In Method 2, pathogen infection was slower but more homogenous. These results demonstrated the suitability of the cultivation system to study the pathosystem soybean/AMF/. We propose this cultivation system for studying the mechanisms involved in the biocontrol conferred by AMF against in soybean.
在这项工作中，我们描述了一种适合研究大豆/丛枝菌根真菌（AMF）/预菌根化幼苗病理系统的体系，预菌根化幼苗通过支撑菌丝体的凝胶块局部感染（方法1）或通过施用于培养基表面的大分生孢子悬浮液感染（方法2）。AMF对根系的定殖和病原体的感染与盆栽试验中的常见观察结果相似。在18天的时间内，超过20%的根系被AMF定殖，并且在所有植株中都观察到了病原体的感染。在有AMF存在的情况下，症状和根系组织感染水平有所降低。采用方法1时，在预菌根化幼苗中观察到较小的坏死病变。采用方法2时，病原体感染较慢但更均匀。这些结果证明了该培养体系适用于研究大豆/AMF/病理系统。我们提出这个培养体系用于研究AMF对大豆中[病原体名称缺失]的生物防治所涉及的机制。
