Motamed Fath Puria, Yazdian Fatemeh, Jamjah Rogayyeh, Ebrahimi Hosseinzadeh Bahman, Rahimnezhad Maede, Sahraeian Razi, Hatamian Ashrafalsadat
Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Iran Polymer and Petrochemical Institute, Tehran, Iran.
Cell J. 2017 Jul-Sep;19(2):269-277. doi: 10.22074/cellj.2016.4119. Epub 2017 Feb 22.
Poly [2-methacryloyloxyethyl phosphoryl choline (MPC)-co-n-buthyl methacrylate (BMA)-co-p-nitrophenyl-oxycrabonyl poly ethylene glycol-methacrylate (ME- ONP)] (PMBN), a biocompatible terpolymer, is a unique polymer with applications that range from drug delivery systems (DDS) to scaffolds and biomedical devices. In this research, we have prepared a monomer of p-nitrophenyl-oxycarbonyl poly (ethylene glycol) methacrylate (MEONP) to synthesize this polymer. Next, we designed and prepared a smart, water soluble, amphiphilic PMBN polymer composed of MPC, BMA, and MEONP.
In this experimental study, we dissolved MPC (4 mmol, 40% mole fraction), BMA (5 mmol, 50% mole fraction), and MEONP (1 mmol, 10% mole fraction) in 20 ml of dry ethanol in two necked flasks equipped with inlet-outlet gas. The structural characteristics of the synthesized monomer and polymer were determined by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (H-NMR), dynamic light scattering (DLS), gel permeation chromatography (GPC), scanning electron microscope (SEM), and transmission electron microscope (TEM) analyses for the first time. We treated the polymer with two different cell lines to determine its biocompatibility.
FT-IR and H-NMR analyses confirmed the synthesis of the polymer. The size of polymer was approximately 40 nm with a molecular weight (MW) of 52 kDa, which would be excellent for a nano carrier. Microscopic analyses showed that the polymer was rodshaped. This polymer had no toxicity for individual cells.
We report here, for the first time, the full properties of the PMBN polymer. The approximately 40 nm size with an acceptable zeta potential range of -8.47, PDI of 0.1, and rod-shaped structure indicated adequate parameters of a nanopolymer for nano bioapplications. We used this polymer to design a new smart nano carrier to treat leukemia stem cells based on a target DDS as a type of bio-application.
聚[2-甲基丙烯酰氧乙基磷酰胆碱（MPC）-共-甲基丙烯酸正丁酯（BMA）-共-对硝基苯氧基羰基聚乙二醇甲基丙烯酸酯（ME-ONP）]（PMBN）是一种生物相容性三元共聚物，是一种独特的聚合物，其应用范围从药物递送系统（DDS）到支架和生物医学装置。在本研究中，我们制备了对硝基苯氧基羰基聚乙二醇甲基丙烯酸酯（MEONP）单体以合成该聚合物。接下来，我们设计并制备了一种由MPC、BMA和MEONP组成的智能、水溶性两亲性PMBN聚合物。
在本实验研究中，我们将MPC（4 mmol，摩尔分数40%）、BMA（5 mmol，摩尔分数50%）和MEONP（1 mmol，摩尔分数10%）溶解在20 ml装有进出气口的两口烧瓶中的无水乙醇中。首次通过傅里叶变换红外光谱（FT-IR）、质子核磁共振（H-NMR）、动态光散射（DLS）、凝胶渗透色谱（GPC）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析确定了合成单体和聚合物的结构特征。我们用两种不同的细胞系处理该聚合物以确定其生物相容性。
FT-IR和H-NMR分析证实了聚合物的合成。聚合物尺寸约为40 nm，分子量（MW）为52 kDa，这对于纳米载体来说是极好的。显微镜分析表明该聚合物呈棒状。该聚合物对单个细胞无毒性。
我们在此首次报告了PMBN聚合物的完整性质。约40 nm的尺寸、-8.47的可接受zeta电位范围、0.1的PDI以及棒状结构表明该纳米聚合物具有适用于纳米生物应用的参数。我们使用这种聚合物设计了一种新型智能纳米载体，基于靶向DDS作为一种生物应用来治疗白血病干细胞。
