Monis B, Valentich M A
J Leukoc Biol. 1986 Feb;39(2):133-40. doi: 10.1002/jlb.39.2.133.
The present study is an electron-microscopic investigation of granuloma induced in the rat by the subcutaneous injection of 1% lambda-carrageenan in 0.8% sodium chloride. We report on the presence of desmosome-like structures connecting neighbouring macrophages. They consisted of electron-dense plaques, which are closely applied to the cytoplasmic surfaces of the junction. Usually, no bundles of cytoplasmic filaments attached to the plaques were seen. At the intercellular space at the level of the junction, a dense material was observed that was sometimes arranged in a cross-bridge fashion. The lysosomal system was markedly developed and consisted mainly of secondary lysosomes containing a filamentous material that showed intense acid phosphatase activity, which was also demonstrated in Golgi cisternae. A membrane preparation obtained from rat carrageenan granuloma contained gamma-glutamyltranspeptidase activity (GGTP), which is a cell and lysosomal membrane-bound enzyme. The GGTP activity of a similar membrane preparation of guinea pig carrageenan granuloma was six times higher than that of the rat.
本研究是一项电子显微镜研究，通过在大鼠皮下注射1%角叉菜胶于0.8%氯化钠溶液中诱导肉芽肿形成。我们报告了连接相邻巨噬细胞的桥粒样结构的存在。它们由电子致密斑块组成，紧密贴附于连接处的细胞质表面。通常，未见附着于斑块的细胞质细丝束。在连接处水平的细胞间空间，观察到一种致密物质，有时呈交叉桥状排列。溶酶体系统明显发达，主要由含有丝状物质的次级溶酶体组成，该丝状物质显示出强烈的酸性磷酸酶活性，高尔基体池也有此活性。从大鼠角叉菜胶肉芽肿获得的膜制剂含有γ-谷氨酰转肽酶活性（GGTP），这是一种细胞和溶酶体膜结合酶。豚鼠角叉菜胶肉芽肿类似膜制剂的GGTP活性比大鼠的高六倍。
