van Dijk Elon H C, Tsonaka Roula, Klar-Mohamad Ngaisah, Wouters Diana, de Vries Aiko P J, de Jong Eiko K, van Kooten Cees, Boon Camiel J F
Department of Ophthalmology, Leiden University Medical Center, Leiden, the Netherlands.
Department of Medical Statistics and Bioinformatics, Leiden University Medical Center, Leiden, the Netherlands.
PLoS One. 2017 Jul 3;12(7):e0180312. doi: 10.1371/journal.pone.0180312. eCollection 2017.
A clear link between several variants in genes involved in the complement system and chronic central serous chorioretinopathy (CSC) has been described. In age-related macular degeneration, a disease that shows clinical features that overlap with CSC, both genetic risk factors and systemic activation of the complement system have previously been found. In this case-control study, we assessed whether there is evidence of either systemic activation or inhibition of the complement system in patients with chronic CSC.
A prospective case-control study of 76 typical chronic CSC patients and 29 controls without ophthalmological history was conducted. Complement activity assays (classical, alternative, and mannose-binding lectin pathway), complement factors 3, 4, 4A, 4B, B, D, H, I, and P, activation products C3d, C5a, and sC5b-C9, and the C3d/C3 ratio were analysed in either serum or plasma. A correction for possible effects of gender, age, body mass index, and smoking status was performed.
In this study, none of the tested variables, including regulation and activation products, proved to be significantly different between the groups. Moreover, no associations with either CSC disease activity or possible CSC related steroid use were observed.
Despite the available literature regarding a possible relationship between chronic CSC and variants in genes involved in the complement system, we did not find evidence of an association of chronic CSC with either systemic complement activation or inhibition.
已有研究描述了补体系统相关基因的几种变异与慢性中心性浆液性脉络膜视网膜病变（CSC）之间存在明确联系。在年龄相关性黄斑变性（一种具有与CSC重叠临床特征的疾病）中，先前已发现遗传风险因素和补体系统的全身激活。在这项病例对照研究中，我们评估了慢性CSC患者是否存在补体系统全身激活或抑制的证据。
对76例典型慢性CSC患者和29例无眼科病史的对照者进行了一项前瞻性病例对照研究。对血清或血浆中的补体活性测定（经典途径、替代途径和甘露糖结合凝集素途径）、补体因子3、4、4A、4B、B、D、H、I和P、激活产物C3d、C5a和sC5b - C9以及C3d/C3比值进行了分析。对性别、年龄、体重指数和吸烟状况的可能影响进行了校正。
在本研究中，包括调节和激活产物在内的所有测试变量在两组之间均无显著差异。此外，未观察到与CSC疾病活动或可能与CSC相关的类固醇使用之间的关联。
尽管有关于慢性CSC与补体系统相关基因变异之间可能关系的现有文献，但我们未发现慢性CSC与全身补体激活或抑制相关的证据。
