Correlation analysis of human embryo Le glycan antigen expression and embryo quality.

This study assessed the feasibility of using Le glycan secretion level in human embryos as a method of judging embryo quality. Embryo culture media from patients receiving fertilization-embryo transfer was collected, and quality scores of embryos were recorded. Secretions of Le in the culture media in different development stages (from 4-cell to 10-cell), embryos in the same development stage of the same patients (8-cell/I) and embryos in the same development stage of different patients (8-cell/I) were examined by dot-blot. Embryos were divided into a hypersecretion group and hyposecretion group, based on their Le secretion level. The embryo quality was evaluated by clinical observations, the number which developed to D3 cell stage and the number of successful embryo transplantations. Le secretion increased as embryos developed from 4-cell to 10-cell (P<0.05); secretion of Le of 8/I is not identical; development speed of embryos with different secretion level of Le was also different. The number of embryos which developed to 6-cell or higher was 82.2% in the Le hypersecretion group but only 60% in the hyposecretion group. The rate of successful transplantation was significantly higher in the hypersecretion group (71.1 vs. 40%). In conclusion, Le glycan secretion level in human embryos is closely related to embryo quality. Le may become a useful measure to evaluate embryo quality in the future.