Fukui Keita, Nanatani Kei, Hara Yoshihiko, Yamakami Suguru, Yahagi Daiki, Chinen Akito, Tokura Mitsunori, Abe Keietsu
a Frontier Research Laboratories , Institute for Innovation, Ajinomoto Co., Inc. , Kawasaki-ku , Japan.
b Department of Microbial Resources, Department of Microbial Biotechnology , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
Biosci Biotechnol Biochem. 2017 Sep;81(9):1837-1844. doi: 10.1080/09168451.2017.1345612. Epub 2017 Jul 4.
Under anaerobic conditions, Escherichia coli produces succinate from glucose via the reductive tricarboxylic acid cycle. To date, however, no genes encoding succinate exporters have been established in E. coli. Therefore, we attempted to identify genes encoding succinate exporters by screening an E. coli MG1655 genome library. We identified the yjjPB genes as candidates encoding a succinate transporter, which enhanced succinate production in Pantoea ananatis under aerobic conditions. A complementation assay conducted in Corynebacterium glutamicum strain AJ110655ΔsucE1 demonstrated that both YjjP and YjjB are required for the restoration of succinate production. Furthermore, deletion of yjjPB decreased succinate production in E. coli by 70% under anaerobic conditions. Taken together, these results suggest that YjjPB constitutes a succinate transporter in E. coli and that the products of both genes are required for succinate export.
在厌氧条件下，大肠杆菌通过还原性三羧酸循环从葡萄糖产生琥珀酸。然而，迄今为止，大肠杆菌中尚未确定编码琥珀酸输出蛋白的基因。因此，我们试图通过筛选大肠杆菌MG1655基因组文库来鉴定编码琥珀酸输出蛋白的基因。我们将yjjPB基因鉴定为编码琥珀酸转运蛋白的候选基因，该基因在有氧条件下增强了菠萝泛菌中琥珀酸的产生。在谷氨酸棒杆菌菌株AJ110655ΔsucE1中进行的互补试验表明，YjjP和YjjB都是恢复琥珀酸产生所必需的。此外，在厌氧条件下，yjjPB的缺失使大肠杆菌中的琥珀酸产量降低了70%。综上所述，这些结果表明YjjPB在大肠杆菌中构成了一种琥珀酸转运蛋白，并且两个基因的产物都是琥珀酸输出所必需的。
