Zou Gang-Ming, Yu Jieqing, LeBron Cynthia, Fu Yumei
Department of Otolaryngology, The First Affiliated Hospital of Nanchang University, Nanchang University, 17 Yong Wai Zheng Street, Nanchang City, 330006, China.
Department of Pathology and Otolaryngology, Johns Hopkins University School of Medicine, 1550 Orleans Street, Baltimore, MD, 21231, USA.
Methods Mol Biol. 2017;1622:131-138. doi: 10.1007/978-1-4939-7108-4_10.
Murine embryonic stem cells (ES) are pluripotent cells and have the potential to become a wide variety of specialized cell types. Mouse ES cell differentiation can be regarded as a valuable biological tool that has led to major advances in our understanding of cell and developmental biology. In vitro differentiation of mouse ES cells can be directed to a specific lineage formation, such as hematopoietic lineage, by appropriate cytokine and/or growth factor stimulation. To study specific gene function in early developmental events, gene knockout approaches have been traditionally used, however, this is a time-consuming and expensive approach. Recently, we have shown that siRNA is an effective strategy to knock down target gene expression, such as Ape1, during ES cell differentiation, and consequently, one can alter cell fates in ES-derived differentiated cells. This approach will be applicable to test the function of a wide variety of gene products using the ES cell differentiation system.
小鼠胚胎干细胞（ES）是多能细胞，有潜力分化为多种特化细胞类型。小鼠胚胎干细胞分化可被视为一种有价值的生物学工具，它在我们对细胞和发育生物学的理解方面带来了重大进展。通过适当的细胞因子和/或生长因子刺激，小鼠胚胎干细胞的体外分化可被引导至特定谱系形成，如造血谱系。为了研究早期发育事件中的特定基因功能，传统上采用基因敲除方法，然而，这是一种耗时且昂贵的方法。最近，我们已经表明，小干扰RNA（siRNA）是在胚胎干细胞分化过程中敲低靶基因表达（如Ape1）的有效策略，因此，可以改变胚胎干细胞来源的分化细胞的细胞命运。这种方法将适用于使用胚胎干细胞分化系统来测试多种基因产物的功能。
