Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
Clin Pharmacol Ther. 2018 Apr;103(4):692-702. doi: 10.1002/cpt.784. Epub 2017 Sep 19.
The advent of mass spectrometry-based analytical technologies coupled with multivariate statistical methods offer tremendous new opportunities for understanding the pharmacokinetics (PKs) of multicomponent herbal medicines (HMs). We recently proposed a poly-PK strategy to characterize the concentration-time profile and the metabolic response profile of multicomponent HMs using an integrated phytochemical and metabolomics approach. Here, we provided the first example of the poly-PK strategy, in which we simultaneously characterized the PK as well as the metabolic response profiles of a Chinese HM, Huangqi decoction (HQD, consisting of Radix Astragali and Radix Glycyrrhizae), in healthy Chinese volunteers. Using the poly-PK approach, we identified 56 HQD-derived compounds and 292 biotransformed HQD metabolites in human plasma. Additionally, we acquired the concentration-time profiles of these plasma HQD metabolites and correlated them with a plasma metabolomics profile consisting of 166 human endogenous metabolites that were significantly altered in response to HQD intervention.
基于质谱分析技术与多元统计方法的联合应用，为深入理解多组分中草药（HM）的药代动力学（PKs）提供了巨大的新机遇。我们最近提出了一种多 PK 策略，采用综合植物化学和代谢组学方法来描述多组分 HM 的浓度-时间曲线和代谢反应谱。在此，我们提供了多 PK 策略的首例实例，该实例同时描述了健康中国志愿者体内一种中草药，黄芪汤（HQD，由黄芪和甘草组成）的 PK 及代谢反应谱。采用多 PK 方法，我们在人血浆中鉴定出 56 种 HQD 衍生化合物和 292 种 HQD 生物转化代谢物。此外，我们获得了这些血浆 HQD 代谢物的浓度-时间曲线，并将其与包含 166 个人内源性代谢物的血浆代谢组学图谱相关联，这些代谢物对 HQD 干预有显著响应。
