Bablanian R, Coppola G, Masters P S, Banerjee A K
Virology. 1986 Jan 30;148(2):375-80. doi: 10.1016/0042-6822(86)90334-x.
Vaccinia virus transcripts synthesized in vitro inhibit protein synthesis directed by globin mRNA, EMC RNA, and total cytoplasmic RNA isolated from HeLa and CHO cells in micrococcal nuclease-treated reticulocyte lysates. In contrast, RNA isolated from vaccinia virus-infected cells is efficiently translated in the same system in the presence of the in vitro transcripts (G. Coppola and R. Bablanian (1983), Proc. Natl. Acad. Sci. USA 80, 75-79). In this study, we have fractionated the in vitro transcripts by acid-urea-agarose gel electrophoresis and demonstrated that the smallest RNA fraction inhibited translation of HeLa cell mRNA in vitro but had no effect on vaccinia virus mRNA translation. On the other hand, the larger RNA species inhibited nonselectively. These results indicate that the selective inhibitory activity of vaccinia virus transcripts resides only in the small poly(A)-containing in vitro RNA fraction.
在微球菌核酸酶处理的网织红细胞裂解物中，体外合成的痘苗病毒转录物可抑制由珠蛋白mRNA、脑心肌炎病毒（EMC）RNA以及从HeLa和CHO细胞中分离的总细胞质RNA所指导的蛋白质合成。相比之下，在体外转录物存在的情况下，从痘苗病毒感染细胞中分离的RNA能在同一系统中高效翻译（G. 科波拉和R. 巴布拉尼安（1983年），《美国国家科学院院刊》80，75 - 79）。在本研究中，我们通过酸性尿素琼脂糖凝胶电泳对体外转录物进行了分级分离，并证明最小的RNA级分在体外抑制HeLa细胞mRNA的翻译，但对痘苗病毒mRNA的翻译没有影响。另一方面，较大的RNA种类则无选择性地抑制。这些结果表明，痘苗病毒转录物的选择性抑制活性仅存在于含小聚腺苷酸的体外RNA级分中。
