利用非整合型仙台病毒从成年食蟹猴皮肤成纤维细胞中生成食蟹猴诱导多能干细胞系ATCi-MF1 。
Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses.
作者信息
Coppiello Giulia, Abizanda Gloria, Aguado Natalia, Iglesias Elena, Arellano-Viera Estibaliz, Rodriguez-Madoz Juan R, Carvajal-Vergara Xonia, Prosper Felipe, Aranguren Xabier L
机构信息
Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
出版信息
Stem Cell Res. 2017 May;21:1-4. doi: 10.1016/j.scr.2017.03.008. Epub 2017 Mar 16.
We generated ATCi-MF1 induced pluripotent stem (iPS) cell line from Macaca fascicularis adult skin fibroblasts using non-integrative Sendai viruses carrying OCT3/4, KLF4, SOX2 and c-MYC. Once established, ATCi-MF1 cells present a normal karyotype, are Sendai virus-free and express pluripotency associated markers. Microsatellite markers analysis confirmed the origin of the iPS cells from the parental fibroblasts. Pluripotency was tested with the in vivo teratoma formation assay. ATCi-MF1 cell line may be a useful primate iPS cell model to test different experimental conditions where the use of human cells can imply ethical issues, as microinjection of pluripotent stem cells in pre-implantational embryos.
我们使用携带OCT3/4、KLF4、SOX2和c-MYC的非整合仙台病毒，从食蟹猴成年皮肤成纤维细胞中生成了ATCi-MF1诱导多能干细胞（iPS）系。一旦建立，ATCi-MF1细胞呈现正常核型，无仙台病毒，并表达多能性相关标志物。微卫星标记分析证实了iPS细胞来源于亲代成纤维细胞。通过体内畸胎瘤形成试验测试了多能性。ATCi-MF1细胞系可能是一个有用的灵长类iPS细胞模型，用于测试不同的实验条件，在这些条件下使用人类细胞可能会涉及伦理问题，例如将多能干细胞显微注射到植入前胚胎中。
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