Uhm Kyung-Ok, Kim So-Jung, Jo Eun Hee, Go Gue Youn, Choi Hye Young, Im Young Sam, Ha Hye-Yeong, Kim Jung-Hyun, Koo Soo Kyung
Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, Republic of Korea.
Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, Republic of Korea.
Stem Cell Res. 2017 May;21:13-15. doi: 10.1016/j.scr.2017.03.009. Epub 2017 Mar 21.
We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts using a Sendai virus (SeV)-based gene delivery method. The generated hiPSC line, KSCBi002-A, has a normal karyotype (46,XY). The pluripotency and differentiation capacity were characterized by comparison with those of a human embryonic stem cell line. This cell line is registered and available from the National Stem Cell Bank, Korea National Institute of Health.
我们使用基于仙台病毒(SeV)的基因递送方法从皮肤成纤维细胞中生成了人诱导多能干细胞(hiPSC)。所生成的hiPSC系KSCBi002-A具有正常的核型(46,XY)。通过与人类胚胎干细胞系进行比较,对其多能性和分化能力进行了表征。该细胞系已在韩国国立卫生研究院国家干细胞库注册并可供使用。