Haase Alexandra, Göhring Gudrun, Martin Ulrich
Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany.
Department of Human Genetics, Hannover Medical School, 30625 Hannover, Germany.
Stem Cell Res. 2017 May;21:71-73. doi: 10.1016/j.scr.2017.03.022. Epub 2017 Mar 31.
Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34 cells, reprogrammed using CytoTune™ Sendai reprogramming vectors. CD34 cell isolation, cultivation, reprogramming and establishment of resulting hiPSC lines were performed under the exclusive usage of animal-derived component-free (ADCF) materials and components.
最近，临床上适用的诱导多能干细胞（iPSC）衍生物生产中的许多障碍和限制已被克服。无转基因的iPSC可以从易于获取的细胞来源（如血液）高效衍生而来。在此，我们描述了使用CytoTune™仙台重编程载体对脐带血来源的CD34细胞进行重编程，从而产生无转基因的人诱导多能干细胞（hiPS细胞）。CD34细胞的分离、培养、重编程以及所得hiPSC系的建立均在无动物源成分（ADCF）材料和成分的专用条件下进行。
