Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.
Oncol Rep. 2017 Aug;38(2):1312-1320. doi: 10.3892/or.2017.5787. Epub 2017 Jul 3.
Matrine is a traditional Chinese herbal medicine that shows antitumor efficacy for many types of cancer. The present study evaluated the antitumor efficacy of matrine on cervical cancer and to investigate the underlying mechanisms. We performed MTT assays, and in vitro invasion and migration assays, and P1 L6 found that matrine significantly inhibited cervical cancer cell growth by inducing apoptosis, and suppressed the invasion and migration ability of cervical cancer cells in vitro in a concentration-dependent manner. Mechanistically, we found that matrine decreased the expression and activity of the extracellular matrix factors, matrix metalloproteinases-2 (MMP-2) and MMP-9 via the suppression of p38 signaling pathway. In addition, when cervical cancer cells were grown as xenografts in nude mice, intraperitoneal (i.p.) injection of matrine induced a significant dose-dependent decrease in tumor growth. Taken together, these findings suggest that a potential mechanism by which matrine inhibits the growth and metastasis of cervical cancer through downregulating the p38 signaling pathway.
苦参碱是一种传统的中草药，对多种癌症具有抗肿瘤功效。本研究评估了苦参碱对宫颈癌的抗肿瘤功效，并探讨了其潜在的机制。我们通过 MTT 分析、体外侵袭和迁移实验发现，苦参碱通过诱导细胞凋亡显著抑制宫颈癌细胞生长，并呈浓度依赖性抑制宫颈癌细胞的体外侵袭和迁移能力。机制上，我们发现苦参碱通过抑制 p38 信号通路降低细胞外基质因子基质金属蛋白酶-2（MMP-2）和 MMP-9 的表达和活性。此外，当宫颈癌细胞在裸鼠中作为异种移植物生长时，苦参碱腹腔注射诱导肿瘤生长显著剂量依赖性下降。综上所述，这些发现表明苦参碱通过下调 p38 信号通路抑制宫颈癌的生长和转移的潜在机制。
