Orsini J C
Brain Res Bull. 1985 Dec;15(6):547-52. doi: 10.1016/0361-9230(85)90203-5.
Direct excitatory effects of sex steroids on lateral hypothalamic-medial forebrain bundle (LHA-MFB) neurons have been demonstrated in a previous study using the microiontophoretic ejection of charged hormone derivatives. In the present study neuronal discharge frequency was recorded during the local pressure ejection of the physiologically active form of these hormones. Of 56 neurones studied, 12 increased in activity due to testosterone application. Twenty-eight of these cells were tested with both testosterone and estradiol. Five were activated by both hormones, 4 were specifically excited by 17 Beta-estradiol, 3 were specifically excited by testosterone, and 16 were unaffected. The short latency of neuronal responses and the efficacy of nanomolar hormonal concentrations suggest the existence of high affinity specific sites in the plasma membrane of responsive neurons. The physiological significance of these neurons is discussed.
在先前一项使用带电激素衍生物微量离子电泳喷射的研究中，已证明性类固醇对下丘脑外侧 - 内侧前脑束（LHA - MFB）神经元有直接兴奋作用。在本研究中，在这些激素的生理活性形式进行局部压力喷射期间记录神经元放电频率。在所研究的56个神经元中，有12个因应用睾酮而活性增加。其中28个细胞同时用睾酮和雌二醇进行测试。5个被两种激素激活，4个被17β - 雌二醇特异性兴奋，3个被睾酮特异性兴奋，16个未受影响。神经元反应的短潜伏期和纳摩尔激素浓度的效力表明在反应性神经元的质膜中存在高亲和力特异性位点。讨论了这些神经元的生理意义。
