Department of Vascular Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
Eur Rev Med Pharmacol Sci. 2017 Jun;21(11):2743-2748.
To study the expression, roles, and clinical significance of Brahma (BRM) and matrix metalloproteinase 2 (MMP2) in the thoracic aortic aneurysm and aortic dissection.
Arterial specimens from 20 cases of thoracic aortic dissection and 38 cases of thoracic aortic aneurysm, as well as normal tissue were collected, paraffin-embedded, sectioned, and stained with anti-BRM and MMP2 monoclonal antibodies. Sections were analyzed by immunofluorescence, and the distribution and expression of BRM and MMP2 in the aortic wall were determined. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the expression of BRM and MMP2 mRNA in the thoracic aortic aneurysm, thoracic aortic dissection, and normal tissues. The expression of MMP2 and BRM protein in these tissues was analyzed by Western blot. SPSS 17.0 statistical software was used for data analysis.
MMP2 and BRM (mRNA and protein) were expressed in arterial tissue from thoracic aortic aneurysms and aortic dissections. Immunofluorescence also showed that BRM and MMP2 were expressed in the thoracic aortic aneurysm and aortic dissection tissue. The expression was very high in thoracic aortic aneurysm tissue. The differences in expression of BRM and MMP2 in the different arterial tissues were statistically significant (p<0.01).
Expression of BRM and MMP2 in the thoracic aortic aneurysm and aortic dissection is very high, indicating that BRM and MMP2 may play important roles in the occurrence and development of thoracic aortic aneurysm and aortic dissection. They may represent potential targets for the treatment of thoracic aortic aneurysm and aortic dissection and provide a new basis for clinical diagnosis.
研究 Brama（BRM）和基质金属蛋白酶 2（MMP2）在胸主动脉瘤和主动脉夹层中的表达、作用及临床意义。
收集 20 例胸主动脉夹层和 38 例胸主动脉瘤的动脉标本及正常组织，石蜡包埋、切片、用抗 BRM 和 MMP2 单克隆抗体染色。通过免疫荧光分析，确定 BRM 和 MMP2 在主动脉壁中的分布和表达。采用逆转录-聚合酶链反应（RT-PCR）测量胸主动脉瘤、胸主动脉夹层和正常组织中 BRM 和 MMP2mRNA 的表达。通过 Western blot 分析这些组织中 MMP2 和 BRM 蛋白的表达。采用 SPSS 17.0 统计软件进行数据分析。
MMP2 和 BRM（mRNA 和蛋白）在胸主动脉瘤和主动脉夹层的动脉组织中表达。免疫荧光也显示 BRM 和 MMP2 在胸主动脉瘤和主动脉夹层组织中表达。胸主动脉瘤组织中的表达非常高。BRM 和 MMP2 在不同动脉组织中的表达差异具有统计学意义（p<0.01）。
BRM 和 MMP2 在胸主动脉瘤和主动脉夹层中的表达非常高，表明 BRM 和 MMP2 可能在胸主动脉瘤和主动脉夹层的发生发展中发挥重要作用。它们可能成为胸主动脉瘤和主动脉夹层治疗的潜在靶点，为临床诊断提供新的依据。
