Metna-Laurent Mathilde, Mondésir Miguel, Grel Agnès, Vallée Monique, Piazza Pier-Vincenzo
Aelis Farma, Neurocentre Magendie, Bordeaux, France.
Physiopathology of Neuronal Plasticity, Neurocentre Magendie, INSERM U1215, Bordeaux, France.
Curr Protoc Neurosci. 2017 Jul 5;80:9.59.1-9.59.10. doi: 10.1002/cpns.31.
Cannabinoid-induced tetrad is a preclinical model commonly used to evaluate if a pharmacological compound is an agonist of the central type-1 cannabinoid (CB1) receptor in rodents. The tetrad is characterized by hypolocomotion, hypothermia, catalepsy, and analgesia, four phenotypes that are induced by acute administration of CB1 agonists exemplified by the prototypic cannabinoid delta-9-tetrahydrocannabinol (THC). This unit describes a standard protocol in mice to induce tetrad phenotypes with THC as reference cannabinoid. We provide typical results obtained with this procedure showing a dose effect of THC in different mouse strains. The effect of the CB1 antagonist rimonabant is also shown. This tetrad protocol is well adapted to reveal new compounds acting on CB1 receptors in vivo. © 2017 by John Wiley & Sons, Inc.
大麻素诱导四联征是一种临床前模型，常用于评估一种药理化合物是否为啮齿动物中枢1型大麻素（CB1）受体的激动剂。该四联征的特征为运动减少、体温过低、僵住症和镇痛，这四种表型由CB1激动剂急性给药诱导产生，以原型大麻素δ-9-四氢大麻酚（THC）为代表。本单元描述了一种以THC作为参考大麻素在小鼠中诱导四联征表型的标准方案。我们提供了通过该程序获得的典型结果，显示了THC在不同小鼠品系中的剂量效应。还展示了CB1拮抗剂利莫那班的作用。这种四联征方案非常适合用于揭示体内作用于CB1受体的新化合物。© 2017约翰威立父子出版公司
