Randazzo Basilio, Chemello Giulia, Tortarolo Ivan, Chiarello Gian Luca, Zalas Maciej, Santini Alessandra, Liberatore Margaret, Liberatore Marco, Selli Elena, Olivotto Ike
1 Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche , Ancona, Italy .
2 Studio Associato Costanzo Tortarolo , Genova, Italy .
Zebrafish. 2017 Oct;14(5):411-421. doi: 10.1089/zeb.2017.1448. Epub 2017 Jul 5.
The accumulation of nitrogen compounds represents a pivotal problem in the management of fish culture. Several methods were investigated in the last decades for treatment of waste waters, and the use of photocatalytic materials has received increasing attention. The photocatalytic degradation (PCD) process with titanium dioxide (TiO) represents the most promising single-step method to promote the removal of nitrogen compounds from water. The present study compares for the first time the effects on fish culture of a classical mechanical, biological, and ultraviolet purification system to a TiO-PCD one, with particular emphasis on water chemistry and on physiological responses in zebrafish. Fish were exposed for 14 days to the two different purification systems and samplings were performed 7 and 14 days after the experiment beginning. The photocatalytic system showed excellent efficiency in removing nitrogen compounds from water with no significant adverse effects on fish. Physiological analysis on fish samples included histological analysis of gills and gut, TUNEL assay of the gills, and real-time polymerase chain reaction (liver) of genes involved in stress response and growth. No significant biological alterations were detectable on the cultured fish.
氮化合物的积累是鱼类养殖管理中的一个关键问题。在过去几十年里，人们研究了几种处理废水的方法，光催化材料的使用受到了越来越多的关注。用二氧化钛（TiO₂）进行光催化降解（PCD）过程是促进从水中去除氮化合物最有前景的单步方法。本研究首次比较了经典的机械、生物和紫外线净化系统与TiO₂-PCD系统对鱼类养殖的影响，特别强调了水化学和斑马鱼的生理反应。将鱼暴露于两种不同的净化系统中14天，并在实验开始后的第7天和第14天进行采样。光催化系统在从水中去除氮化合物方面表现出优异的效率，且对鱼没有明显的不利影响。对鱼样本的生理分析包括鳃和肠道的组织学分析、鳃的TUNEL检测以及肝脏中参与应激反应和生长的基因的实时聚合酶链反应。在养殖鱼身上未检测到明显的生物学变化。
