Wehrenberg W B
Endocrinology. 1986 Feb;118(2):489-94. doi: 10.1210/endo-118-2-489.
The role of GH-releasing factor (GRF) in regulating somatic growth was studied in rats by passively immunizing animals with antisera raised against rat GRF. GRF antiserum (GRF-ab) treatment significantly inhibited the normal increase in body weight observed in both young male and female rats. Similar studies conducted in neonatal rats (days 1-23 of age) also demonstrated that GRF is critical in regulating neonatal growth. The biological half-life of the GRF-ab was determined to be between 2.5 and 3.5 days in these animals. The effects of GRF-ab and somatostatin antiserum (SRIF-ab) treatment on serum GH concentrations were also studied in neonatal rats. Between 1 and 20 days of age, GRF-ab treatment significantly decreased serum GH concentrations compared to those in control animals. From days 1-10 of age, SRIF-ab treatment had no effect on GH concentrations, whereas on days 15 and 20 of age, SRIF-ab treatment significantly increased GH concentrations. The results demonstrate that hypothalamic GRF is critical in regulating serum GH concentrations and somatic growth in neonatal and young animals. They also suggest that the role of SRIF in regulating GH concentrations is not established until sometime after 10 days of age.
通过用抗大鼠生长激素释放因子（GRF）的抗血清被动免疫大鼠，研究了GRF在调节大鼠体细胞生长中的作用。GRF抗血清（GRF-ab）处理显著抑制了年轻雄性和雌性大鼠体重的正常增加。在新生大鼠（1至23日龄）中进行的类似研究也表明，GRF在调节新生大鼠生长中起关键作用。在这些动物中，GRF-ab的生物半衰期被确定为2.5至3.5天。还在新生大鼠中研究了GRF-ab和生长抑素抗血清（SRIF-ab）处理对血清生长激素（GH）浓度的影响。在1至20日龄之间，与对照动物相比，GRF-ab处理显著降低了血清GH浓度。在1至10日龄时，SRIF-ab处理对GH浓度没有影响，而在15和20日龄时，SRIF-ab处理显著增加了GH浓度。结果表明，下丘脑GRF在调节新生和幼年动物的血清GH浓度和体细胞生长中起关键作用。它们还表明，SRIF在调节GH浓度中的作用直到10日龄后的某个时间才得以确立。
