Tuken Murat, Temiz Mustafa Zafer, Yuruk Emrah, Kaptanagasi Asuman Orcun, Basak Kayhan, Narter Fehmi, Muslumanoglu Ahmet Yaser, Sarica Kemal
Bagcilar Training & Research Hospital, Dept. of Urology, Istanbul.
Arch Ital Urol Androl. 2017 Jun 30;89(2):134-138. doi: 10.4081/aiua.2017.2.134.
The aim of this study is to evaluate the effects of the herbal agent in the prevention and treatment of bacterial cystitis in a rat model.
A total of twenty-eight male Sprague- Dawley rats were divided into four groups. Group-1 constituted the control group (operated and normal saline injected into the bladder, received only drinking water for 7 days); Group-2 constituted the no-treatment group (operated, E.coli J96 strain injected into the bladder, received only drinking water for 7 days); Group-3 constituted the short-term treatment (operated, E.coli J96 strain injected into the bladder, received the herbal agent added into drinking water for 7 days) and Group-4 constituted the long-term treatment (operated, E. coli J96 strain injected into the bladder, received herbal agent added into drinking water for 14 days). At the end of the pre-defined treatment periods of duration, the rats were sacrificed, urine samples collected from the bladder for culture and bladders were harvested for histopathological evaluation. Urine culture results and histopathological findings were comparatively evaluated between the groups.
Urine cultures were positive for implanted E. coli strains in 0%, 85.7%, 42.8% and 0% of rats in Group 1, Group 2, Group 3 and Group 4, respectively (p = 0.001). Although histopathological evaluation revealed increased vascular dilation in the bladder specimens obtained from Group 2 and Group 3 (p = 0.028) no significant difference was noticed in level of inflammation (p = 0.610), edema (p = 0.754) and thickness of uroepithelium (p = 0.138).
While long term (14 days) treatment with an herbal agent added into the drinking water resulted in complete clearance of urine from E. coli; shorter application of the agent revealed partial clearance. Further clinical studies are needed to support our results.
本研究旨在评估一种草药制剂在大鼠模型中预防和治疗细菌性膀胱炎的效果。
总共28只雄性斯普拉格-道利大鼠被分为四组。第1组为对照组（进行手术并向膀胱注射生理盐水，仅饮用7天的水）；第2组为未治疗组（进行手术，向膀胱注射大肠杆菌J96菌株，仅饮用7天的水）；第3组为短期治疗组（进行手术，向膀胱注射大肠杆菌J96菌株，饮用添加了草药制剂的水7天），第4组为长期治疗组（进行手术，向膀胱注射大肠杆菌J96菌株，饮用添加了草药制剂的水14天）。在预定的治疗期结束时，处死大鼠，收集膀胱尿液样本进行培养，并摘取膀胱进行组织病理学评估。对各组之间的尿液培养结果和组织病理学发现进行比较评估。
第1组、第2组、第3组和第4组大鼠中，植入的大肠杆菌菌株尿液培养阳性率分别为0%、85.7%、42.8%和0%（p = 0.001）。虽然组织病理学评估显示，从第2组和第3组获得的膀胱标本中血管扩张增加（p = 0.028），但在炎症水平（p = 0.610）、水肿（p = 0.754）和尿上皮厚度（p = 0.138）方面未观察到显著差异。
饮用水中添加草药制剂进行长期（14天）治疗可使尿液中大肠杆菌完全清除；而该制剂较短时间的应用显示为部分清除。需要进一步的临床研究来支持我们的结果。
