Dhondt Ineke, Petyuk Vladislav A, Bauer Sophie, Brewer Heather M, Smith Richard D, Depuydt Geert, Braeckman Bart P
From the ‡Laboratory for Aging Physiology and Molecular Evolution, Biology Department, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent Belgium.
§Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Mol Cell Proteomics. 2017 Sep;16(9):1621-1633. doi: 10.1074/mcp.RA117.000049. Epub 2017 Jul 5.
Protein turnover rates severely decline in aging organisms, including However, limited information is available on turnover dynamics at the individual protein level during aging. We followed changes in protein turnover at one-day resolution using a multiple-pulse N-labeling and accurate mass spectrometry approach. Forty percent of the proteome shows gradual slowdown in turnover with age, whereas only few proteins show increased turnover. Decrease in protein turnover was consistent for only a minority of functionally related protein subsets, including tubulins and vitellogenins, whereas randomly diverging turnover patterns with age were the norm. Our data suggests increased heterogeneity of protein turnover of the translation machinery, whereas protein turnover of ubiquitin-proteasome and antioxidant systems are well-preserved over time. Hence, we presume that maintenance of quality control mechanisms is a protective strategy in aging worms, although the ultimate proteome collapse is inescapable.
蛋白质周转率在衰老生物体中会严重下降，包括……然而，关于衰老过程中单个蛋白质水平的周转率动态变化的信息有限。我们使用多脉冲N标记和精确质谱方法，以一天的分辨率跟踪蛋白质周转率的变化。蛋白质组的40%显示周转率随年龄逐渐减慢，而只有少数蛋白质显示周转率增加。蛋白质周转率的下降仅在少数功能相关的蛋白质亚组中一致，包括微管蛋白和卵黄蛋白原，而随着年龄随机变化的周转率模式才是常态。我们的数据表明翻译机制的蛋白质周转率异质性增加，而泛素-蛋白酶体和抗氧化系统的蛋白质周转率随时间保持良好。因此，我们推测维持质量控制机制是衰老蠕虫中的一种保护策略，尽管最终的蛋白质组崩溃是不可避免的。
