Ekwall Karl, Thon Geneviève
Department of Biosciences and Nutrition, Karolinska Institute, Stockholm SE-141 83, Sweden;
Department of Biology, University of Copenhagen, Copenhagen DK-2200, Denmark.
Cold Spring Harb Protoc. 2017 Jul 5;2017(7):pdb.prot091702. doi: 10.1101/pdb.prot091702.
Here we describe procedures for the selection of diploid heteroallelic complementation is widely used to select for Ade diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these cases mating an strain with an strain can be used to prevent sporulation. When mutations impact on, or show synthetic interactions with, the gene of interest, two different auxotrophic markers can be used to select complementation.
在这里，我们描述了选择二倍体的程序。异源等位基因互补被广泛用于选择腺嘌呤二倍体。当缺乏氮时，这种二倍体很容易形成孢子。对于一些研究来说，稳定的二倍体更可取（例如，用于基因互补测试），在这些情况下，可以将一个菌株与另一个菌株交配以防止形成孢子。当突变影响或与感兴趣的基因表现出合成相互作用时，可以使用两种不同的营养缺陷型标记来选择互补。
