Selecting Diploids.

Here we describe procedures for the selection of diploid heteroallelic complementation is widely used to select for Ade diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these cases mating an strain with an strain can be used to prevent sporulation. When mutations impact on, or show synthetic interactions with, the gene of interest, two different auxotrophic markers can be used to select complementation.