Yasuda Masahiko, Ogura Tomoyuki, Goto Takayuki, Yagoto Mika, Kamai Yoko, Shimomura Chie, Hayashimoto Nobuhito, Kiyokawa Yukito, Shinohara Hideki, Takahashi Riichi, Kawai Kenji
Pathology Analysis Center, Central Institute for Experimental Animals (CIEA), 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan.
Animal Resources Center, Central Institute for Experimental Animals (CIEA), 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan.
Exp Anim. 2017 Oct 30;66(4):425-435. doi: 10.1538/expanim.17-0034. Epub 2017 Jul 6.
Severely immunodeficient NOD/Shi-scid, IL-2Rγ (NOG) mice provide an in vivo model for human cell/tissue transplantation studies. NOG mice were established by combining interleukin-2 receptor-γ chain knockout mice and NOD/Shi-scid mice. They exhibit a high incidence of thymic lymphomas and immunoglobulin (Ig) leakiness. In this study, we assessed the incidence of malignant lymphomas and the occurrence of leakiness in 2,184 non-experimental NOG retired breeder mice aged 16-40 weeks. We established that the total incidence of lymphomas was only 0.60% (13/2,184). Most lymphomas (10/13) occurred in female mice by the age of around 25 weeks. No mice developed Ig leakiness. All lymphomas were derived from the thymus, and consisted mainly of CD3-positive and CD45R-negative lymphoblastic-like cells. Therefore, based on the absence of Ig leakiness and a very low incidence of lymphomas, including thymic lymphomas, NOG mice may be useful in regeneration medicine for xenotransplantation of human embryonic stem (ES) cells or induced pluripotent stem (iPS) cells, and in transplantation experiments involving tumor cells.
严重免疫缺陷的NOD/Shi-scid、IL-2Rγ（NOG）小鼠为人类细胞/组织移植研究提供了一种体内模型。NOG小鼠是通过将白细胞介素-2受体γ链敲除小鼠与NOD/Shi-scid小鼠杂交培育而成。它们胸腺淋巴瘤的发生率较高，且存在免疫球蛋白（Ig）渗漏现象。在本研究中，我们评估了2184只16至40周龄的非实验性NOG退休繁殖小鼠的恶性淋巴瘤发生率和渗漏情况。我们确定淋巴瘤的总发生率仅为0.60%（13/2184）。大多数淋巴瘤（10/13）发生在约25周龄的雌性小鼠中。没有小鼠出现Ig渗漏。所有淋巴瘤均起源于胸腺，主要由CD3阳性和CD45R阴性的淋巴母细胞样细胞组成。因此，基于不存在Ig渗漏以及包括胸腺淋巴瘤在内的淋巴瘤发生率极低的情况，NOG小鼠可能在人类胚胎干细胞（ES）或诱导多能干细胞（iPS）的异种移植再生医学以及涉及肿瘤细胞的移植实验中有用。
