Kumar Navin, Shariq Mohd, Kumar Amarjeet, Kumari Rajesh, Subbarao Naidu, Tyagi Rakesh K, Mukhopadhyay Gauranga
Special Centre for Molecular Medicine Jawaharlal Nehru University New Delhi India.
Present address: School of Biotechnology Gautam Buddha University Yamuna Expressway Greater Noida Gautam Budh Nagar Uttar Pradesh India.
FEBS Open Bio. 2017 May 24;7(7):915-933. doi: 10.1002/2211-5463.12225. eCollection 2017 Jul.
The type IV secretion system of (Cag-T4SS) is composed of ~ 27 components including a VirB8 homolog, CagV. We have characterized CagV and reported that it is an inner membrane protein and, like VirB8, forms a homodimer. Its stability is not dependent on the other Cag components and the absence of affects the stability of only CagI, a protein involved in pilus formation. CagV is not required for the stability and localization of outer membrane subcomplex proteins, but interacts with them through CagX. It also interacts with the inner membrane-associated components, CagF and CagZ, and is required for the surface localization of CagA. The results of this study might help in deciphering the mechanistic contributions of CagV in the Cag-T4SS biogenesis and function.
幽门螺杆菌的IV型分泌系统（Cag-T4SS）由约27种成分组成，包括一种VirB8同源物CagV。我们已经对CagV进行了表征，并报道它是一种内膜蛋白，并且与VirB8一样，形成同二聚体。其稳定性不依赖于其他Cag成分，而缺失幽门螺杆菌仅影响CagI（一种参与菌毛形成的蛋白质）的稳定性。CagV对于外膜亚复合体蛋白的稳定性和定位不是必需的，但通过CagX与它们相互作用。它还与内膜相关成分CagF和CagZ相互作用，并且是CagA表面定位所必需的。这项研究的结果可能有助于阐明CagV在Cag-T4SS生物发生和功能中的机制贡献。
