Keikha N, Ayatollahi Mousavi S A, Shahidi Bonjar G H, Fouladi B, Izadi A R
MSc in Medical Mycology, Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Laboratory Associate Professor in Medical Mycology, Infectious Diseases and Tropical Medicine Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Curr Med Mycol. 2015 Sep;1(3):33-38. doi: 10.18869/acadpub.cmm.1.3.33.
Cutaneous infections arise from a homogeneous group of keratinophilic fungi, known as dermatophytes. Since these pathogenic dermatophytes are eukaryotes in nature, use of chemical antifungal agents for treatment may affect the host tissue cells. In this study, we aimed to evaluate the antifungal activity of species against (abbreviated as ). The isolates were obtained from soil samples and identified by polymerase chain reaction (PCR) technique.
In total, 100 strains of species were isolated from soil samples in order to determine their antagonistic activities against in Kerman, Iran The electron microscopic study of these isolates was performed, based on the physiological properties of these antagonists (e.g., lipase, amylase, protease and chitinase), using relevant protocols. The isolates were identified using gene 16S rDNA via PCR technique.
, strain xsd08149 and were isolated and exhibited the most significant antagonistic activities against . Images were obtained by an electron microscope and some spores, mycelia and morphology of spore chains were identified. Molecular, morphological and biochemical characteristics of these isolates were studied, using the internal 16S rDNA gene. Active isolates of sequence were compared to GenBank sequences. According to nucleotide analysis, isolate D5 had maximum similarity to (99%).
The findings of this study showed that isolates from soil samples could exert antifungal effects on .
皮肤感染由一组统称为皮肤癣菌的嗜角质真菌引起。由于这些致病性皮肤癣菌本质上是真核生物，使用化学抗真菌剂进行治疗可能会影响宿主组织细胞。在本研究中，我们旨在评估某物种对[具体名称，文中未给出全称，用缩写表示]的抗真菌活性。这些分离株从土壤样本中获得，并通过聚合酶链反应（PCR）技术进行鉴定。
为了确定从伊朗克尔曼的土壤样本中分离出的总共100株某物种对[具体名称，文中未给出全称，用缩写表示]的拮抗活性，基于这些拮抗剂的生理特性（如脂肪酶、淀粉酶、蛋白酶和几丁质酶）采用相关方案对这些分离株进行了电子显微镜研究。通过PCR技术利用16S rDNA基因对分离株进行鉴定。
分离出了[菌株名称，文中未给出全称，用缩写表示]、菌株xsd08149和[具体名称，文中未给出全称，用缩写表示]，它们对[具体名称，文中未给出全称，用缩写表示]表现出最显著的拮抗活性。通过电子显微镜获得了图像，并鉴定了一些孢子、菌丝体和孢子链的形态。利用内部16S rDNA基因研究了这些分离株的分子、形态和生化特征。将具有活性的[具体名称，文中未给出全称，用缩写表示]序列的分离株与GenBank序列进行了比较。根据核苷酸分析，分离株D5与[具体名称，文中未给出全称，用缩写表示]的相似度最高（99%）。
本研究结果表明，从土壤样本中分离出的[具体名称，文中未给出全称，用缩写表示]分离株可对[具体名称，文中未给出全称，用缩写表示]发挥抗真菌作用。
