antifungal activities of species isolated from soil samples against .

BACKGROUND AND PURPOSE

Cutaneous infections arise from a homogeneous group of keratinophilic fungi, known as dermatophytes. Since these pathogenic dermatophytes are eukaryotes in nature, use of chemical antifungal agents for treatment may affect the host tissue cells. In this study, we aimed to evaluate the antifungal activity of species against (abbreviated as ). The isolates were obtained from soil samples and identified by polymerase chain reaction (PCR) technique.

MATERIAL AND METHODS

In total, 100 strains of species were isolated from soil samples in order to determine their antagonistic activities against in Kerman, Iran The electron microscopic study of these isolates was performed, based on the physiological properties of these antagonists (e.g., lipase, amylase, protease and chitinase), using relevant protocols. The isolates were identified using gene 16S rDNA via PCR technique.

RESULTS

, strain xsd08149 and were isolated and exhibited the most significant antagonistic activities against . Images were obtained by an electron microscope and some spores, mycelia and morphology of spore chains were identified. Molecular, morphological and biochemical characteristics of these isolates were studied, using the internal 16S rDNA gene. Active isolates of sequence were compared to GenBank sequences. According to nucleotide analysis, isolate D5 had maximum similarity to (99%).

CONCLUSION

The findings of this study showed that isolates from soil samples could exert antifungal effects on .