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固体脂质颗粒稳定的油包水乳状液的制备、表征和稳定性:颗粒特性和乳液微结构对 Pickering 功能的作用。

Fabrication, characterisation and stability of oil-in-water emulsions stabilised by solid lipid particles: the role of particle characteristics and emulsion microstructure upon Pickering functionality.

机构信息

School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT, UK.

出版信息

Food Funct. 2017 Jul 19;8(7):2583-2591. doi: 10.1039/c7fo00559h.

Abstract

The quest to identify and use bio-based particles with a Pickering stabilisation potential for food applications has lately been particularly substantial and includes, among other candidates, lipid-based particles. The present study investigates the ability of solid lipid particles to stabilise oil-in-water (o/w) emulsions against coalescence. Results obtained showed that emulsion stability could be achieved when low amounts (0.8 wt/wt%) of a surface active species (e.g. Tween 80 or NaCas) were used in particles' fabrication. Triple staining of the o/w emulsions enabled the visualisation of emulsion droplets' surface via confocal microscopy. This revealed an interfacial location of the lipid particles, hence confirming stabilisation via a Pickering mechanism. Emulsion droplet size was controlled by varying several formulation parameters, such as the type of the lipid and surface active component, the processing route and the polarity of the dispersed phase. Differential scanning calorimetry (DSC) was employed as the analytical tool to quantify the amount of crystalline material available to stabilise the emulsion droplets at different intervals during the experimental timeframe. Dissolution of lipid particles in the oil phase was observed and evolved distinctly between a wax and a triglyceride, and in the presence of a non-ionic surfactant and a protein. Yet, this behaviour did not result in emulsion destabilisation. Moreover, emulsion's thermal stability was found to be determined by the behaviour of lipid particles under temperature effects.

摘要

最近,人们一直在努力寻找和利用具有 Pickering 稳定潜力的生物基颗粒,用于食品应用,其中包括脂质基颗粒等候选物质。本研究调查了固体脂质颗粒稳定油包水(o/w)乳液防止聚结的能力。研究结果表明,当使用少量(0.8wt/wt%)表面活性剂(如吐温 80 或 NaCas)制造颗粒时,可以实现乳液的稳定性。o/w 乳液的三重染色能够通过共聚焦显微镜可视化乳液液滴的表面。这证实了脂质颗粒的界面位置,从而通过 Pickering 机制证实了稳定作用。通过改变多种配方参数(例如脂质和表面活性剂的类型、处理路线和分散相的极性)来控制乳液液滴的大小。差示扫描量热法(DSC)被用作分析工具,以量化在实验时间内不同时间点可用的结晶物质的量,以稳定乳液液滴。观察到脂质颗粒在油相中的溶解,并在存在非离子表面活性剂和蛋白质时,在蜡和甘油三酯之间以及在存在非离子表面活性剂和蛋白质时明显演变。然而,这种行为并没有导致乳液失稳。此外,还发现乳液的热稳定性取决于脂质颗粒在温度影响下的行为。

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