Ezura Kentaro, Ji-Seong Kim, Mori Kazuki, Suzuki Yutaka, Kuhara Satoru, Ariizumi Tohru, Ezura Hiroshi
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
PLoS One. 2017 Jul 6;12(7):e0180003. doi: 10.1371/journal.pone.0180003. eCollection 2017.
Fruit set involves the developmental transition of an unfertilized quiescent ovary in the pistil into a fruit. While fruit set is known to involve the activation of signals (including various plant hormones) in the ovary, many biological aspects of this process remain elusive. To further expand our understanding of this process, we identified genes that are specifically expressed in tomato (Solanum lycopersicum L.) pistils during fruit set through comprehensive RNA-seq-based transcriptome analysis using 17 different tissues including pistils at six different developmental stages. First, we identified 532 candidate genes that are preferentially expressed in the pistil based on their tissue-specific expression profiles. Next, we compared our RNA-seq data with publically available transcriptome data, further refining the candidate genes that are specifically expressed within the pistil. As a result, 108 pistil-specific genes were identified, including several transcription factor genes that function in reproductive development. We also identified genes encoding hormone-like peptides with a secretion signal and cysteine-rich residues that are conserved among some Solanaceae species, suggesting that peptide hormones may function as signaling molecules during fruit set initiation. This study provides important information about pistil-specific genes, which may play specific roles in regulating pistil development in relation to fruit set.
坐果涉及雌蕊中未受精的静止子房向果实的发育转变。虽然已知坐果涉及子房内信号（包括各种植物激素）的激活，但这一过程的许多生物学方面仍不清楚。为了进一步扩展我们对这一过程的理解，我们通过基于RNA测序的转录组分析，使用包括六个不同发育阶段的雌蕊在内的17种不同组织，鉴定了番茄（Solanum lycopersicum L.）雌蕊在坐果期间特异性表达的基因。首先，我们根据532个候选基因的组织特异性表达谱，鉴定出它们在雌蕊中优先表达。接下来，我们将我们的RNA测序数据与公开可用的转录组数据进行比较，进一步筛选出在雌蕊中特异性表达的候选基因。结果，鉴定出108个雌蕊特异性基因，包括几个在生殖发育中起作用的转录因子基因。我们还鉴定出编码具有分泌信号和富含半胱氨酸残基的类激素肽的基因，这些残基在一些茄科物种中是保守的，这表明肽激素可能在坐果起始过程中作为信号分子发挥作用。这项研究提供了有关雌蕊特异性基因的重要信息，这些基因可能在调节与坐果相关的雌蕊发育中发挥特定作用。
