Medical Materials Laboratory, Indian Institute of Technology Madras, Chennai, 600036, India.
Tissue Culture Laboratory, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Trivandrum, 695 012, India.
J Mater Sci Mater Med. 2017 Aug;28(8):119. doi: 10.1007/s10856-017-5933-4. Epub 2017 Jul 6.
Critical size defects in the craniofacial region can be effectively treated using three dimensional (3D) composite structures mimicking natural extra cellular matrix (ECM) and incorporated with bioactive ceramics. In this study we have shown that the dynamic liquid bath collector can be used to form electrospun polycaprolactone (PCL)-hydroxyapatite (HA) composite structure as unique 3D scaffold. The structure was found to have three distinct sections (base, stem and head) based on the mechanism of its formation and morphology. The size of the head portion was around 15 mm and was found to vary with the process parameters. Scanning electron microscopy (SEM) analysis revealed that the base had random fibres while the fibres in stem and head sections were aligned but perpendicular to each other. X-ray diffraction (XRD) analysis also showed an increase in the crystallinity index of the fibres from base to head section. Cytotoxicity and cytocompatibility studies using human osteosarcoma (HOS) cells showed good cell adhesion and proliferation indicating the suitability of the 3D structure for craniofacial graft applications.
颅面部临界尺寸缺陷可以通过模拟天然细胞外基质 (ECM) 的三维 (3D) 复合材料结构并与生物活性陶瓷结合来有效治疗。在这项研究中，我们已经表明，动态液体浴收集器可用于形成电纺聚己内酯 (PCL)-羟基磷灰石 (HA) 复合结构作为独特的 3D 支架。根据其形成机制和形态，发现该结构具有三个不同的部分（基底、茎和头）。头部部分的大小约为 15mm，并且发现其尺寸随工艺参数而变化。扫描电子显微镜 (SEM) 分析显示，基底具有随机纤维，而茎和头部部分的纤维则彼此对齐但相互垂直。X 射线衍射 (XRD) 分析还表明，纤维的结晶度指数从基底到头部部分逐渐增加。使用人骨肉瘤 (HOS) 细胞进行的细胞毒性和细胞相容性研究表明，良好的细胞黏附和增殖表明 3D 结构适合颅面移植物应用。
