乘坐电梯需要两个人。
It takes two to transport via an elevator.
作者信息
Byrne Bernadette
机构信息
Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
出版信息
Cell Res. 2017 Aug;27(8):965-966. doi: 10.1038/cr.2017.89. Epub 2017 Jul 7.
Abstract
Membrane transporter proteins are critical for cellular uptake and export of molecules, and are reported to function by a number of different molecular mechanisms. The new occluded state structure of the uracil transporter, UraA, from Escherichia coli, reveals that both coordinated movement of the two domains of a single protomer together with dimer formation are important for transport activity.
摘要
膜转运蛋白对于细胞摄取和输出分子至关重要，据报道其通过多种不同的分子机制发挥作用。来自大肠杆菌的尿嘧啶转运蛋白UraA的新的封闭态结构表明，单个原体的两个结构域的协同运动以及二聚体形成对于转运活性都很重要。
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Nature. 2017 May 4;545(7652):66-70. doi: 10.1038/nature22057. Epub 2017 Apr 17.
2
Structure of eukaryotic purine/H(+) symporter UapA suggests a role for homodimerization in transport activity.真核嘌呤/H(+) 同向转运体UapA的结构表明同二聚化在转运活性中起作用。
Nat Commun. 2016 Apr 18;7:11336. doi: 10.1038/ncomms11336.
3
Band 3, the human red cell chloride/bicarbonate anion exchanger (AE1, SLC4A1), in a structural context.在结构背景下的带3，即人类红细胞氯/碳酸氢根阴离子交换蛋白（AE1，SLC4A1）。
Biochim Biophys Acta. 2016 Jul;1858(7 Pt A):1507-32. doi: 10.1016/j.bbamem.2016.03.030. Epub 2016 Apr 6.
4
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Annu Rev Biochem. 2016 Jun 2;85:543-72. doi: 10.1146/annurev-biochem-060815-014520. Epub 2016 Mar 21.
5
Crystal structure of the anion exchanger domain of human erythrocyte band 3.人红细胞带 3 阴离子交换结构域的晶体结构。
Science. 2015 Nov 6;350(6261):680-4. doi: 10.1126/science.aaa4335.
6
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7
The SLC26 gene family of anion transporters and channels.阴离子转运体和通道的 SLC26 基因家族。
Mol Aspects Med. 2013 Apr-Jun;34(2-3):494-515. doi: 10.1016/j.mam.2012.07.009.
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Nature. 2009 Dec 17;462(7275):880-5. doi: 10.1038/nature08616. Epub 2009 Nov 18.
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