a Physiology Department , College of Medicine, Center of Excellence in Thrombosis & Hemostasis, King Saud University , Riyadh , Kingdom of Saudi Arabia.
b Clinical and Chemical Pathology Department, Faculty of Medicine , Suez Canal University , Ismailia , Egypt.
Clin Exp Hypertens. 2017;39(8):764-768. doi: 10.1080/10641963.2017.1324481. Epub 2017 Jul 7.
This study was conducted to test the association between promoter DNA methylation of α-Adducin (ADD1) gene and the risk of essential hypertension (EH). A total of 150 EH patients and 100 aged- and gender-matched controls were investigated. DNA methylation levels of five cytosine-phosphate-guanine (CpG) dinucleotides on ADD1 promoter were measured employing bisulfite pyrosequencing technology. Our results showed that females have a higher ADD1 DNA methylation than males and a significantly lower CpG1 methylation level is associated with increased risk of EH among them. As for males, a significant association between lower CpG2-5 methylation levels and increased risk of EH was shown. In addition, CpG2-5 methylation was found to be a highly significant predictor for EH among males. In females, CpG1 methylation was considered a predictor of hypertension. No significant correlations were found with biochemical measures, apart from the concentration of aspartate aminotransferase which was inversely correlated with ADD1 CpG2-5 methylation levels among female controls (r = -0.703). These findings highlight that ADD1 methylation may have a contributing role in the pathogenesis of EH with varying implications for both genders.
本研究旨在检验α-辅肌动蛋白（ADD1）基因启动子 DNA 甲基化与原发性高血压（EH）风险之间的关联。共纳入 150 例 EH 患者和 100 名年龄和性别匹配的对照者。采用亚硫酸氢盐焦磷酸测序技术检测 ADD1 启动子 5 个胞嘧啶-磷酸-鸟嘌呤（CpG）二核苷酸的 DNA 甲基化水平。我们的结果表明，女性的 ADD1 DNA 甲基化水平高于男性，且 CpG1 甲基化水平显著降低与女性 EH 风险增加相关。对于男性，CpG2-5 甲基化水平降低与 EH 风险增加之间存在显著关联。此外，CpG2-5 甲基化是男性 EH 的一个高度显著的预测因子。在女性中，CpG1 甲基化被认为是高血压的一个预测因子。除了天门冬氨酸氨基转移酶浓度与女性对照组 ADD1 CpG2-5 甲基化水平呈负相关（r=-0.703）外，与其他生化指标均无显著相关性。这些发现提示 ADD1 甲基化可能在 EH 的发病机制中起作用，且对两性的影响不同。
