Walter M, Müller J M, Keller H W, Brenner U
Aktuelle Traumatol. 1985 Dec;15(6):254-9.
Native collagen type I was studied morphologically and fluorescent-histologically after implantation in bony defects. As criteria for revitalisation we used depth and density of immigration, type of immigrated cells, revascularisation, formation of new cartilage and bone. Furthermore the deposition of fluorochromes was studied. The maximum of cellular immigration was reached after 8 weeks and remained at this level for the period of observation. The implants were impregnated only with fibroblasts and fibrocytes, developing into chondroblasts, chondrocytes, osteoblasts and osteocytes. Only in one case basophilic round-cells could be seen. The centres of the implants were after 6 weeks rarely, after 8 weeks fully revascularized. Formation of new cartilage and bone could be seen after 6 weeks, increasing in number and extension during the observation-period. Osteoneogenesis was performed both by desmal and enchondral ossification, enchondral ossification much more in evidence. The deposition of fluorochromes could be seen in each implant. After 8 weeks fluorochromes could only be seen at the bone-implant interface, after 12 and 16 weeks even the centres were well impregnated. In a single case reossification in a control-rib could be seen as well morphologically as fluorescent-histologically.
对植入骨缺损后的天然I型胶原蛋白进行了形态学和荧光组织学研究。作为活力恢复的标准，我们采用了细胞迁移的深度和密度、迁移细胞的类型、血管再生、新软骨和骨的形成。此外，还研究了荧光染料的沉积情况。细胞迁移在8周后达到最大值，并在观察期内保持在该水平。植入物仅被成纤维细胞和纤维细胞浸润，并发育成软骨细胞、软骨细胞、成骨细胞和骨细胞。仅在1例中可见嗜碱性圆形细胞。植入物的中心在6周后很少有血管再生，8周后完全血管再生。6周后可见新软骨和骨的形成，在观察期内数量和范围不断增加。骨生成通过膜内成骨和软骨内成骨进行，软骨内成骨更为明显。在每个植入物中都可以看到荧光染料的沉积。8周后，荧光染料仅在骨-植入物界面可见，12周和16周后，甚至中心也被充分浸润。在1例对照肋骨中，在形态学和荧光组织学上均可见再骨化。
