González Javier, Mouhbi Rabiaa, Guevara-González Jesús Alberto, Arroyo José María
Departamento de Producción Agraria, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Ciudad Universitaria, Madrid, Spain.
J Sci Food Agric. 2018 Feb;98(3):891-895. doi: 10.1002/jsfa.8534. Epub 2017 Aug 17.
In situ estimates of ruminally undegraded protein (RUP) and intestinally digested protein (IDP) of ten concentrates, uncorrected or corrected for the ruminal microbial colonization, were used to examine the effects of this correction on the relationship between IDP and RUP values. Both variables were established for three rumen and duodenum cannulated wethers using N labeling-techniques and considering measured rates of ruminal particle comminution (k ) and outflow (k ).
A covariance analysis showed that the close relationship found between both variables (IDP = -0.0132 ± 0.00679 + 0.776 ± 0.0002 RUP; n = 60; P < 0.001; r = 0.960) is not affected by correcting for microbial colonization (P = 0.682).
The IDP content in concentrates and industrial by-products can be predicted from RUP values, thus avoiding the laborious and complex procedure of determining intestinal digestibility; however, a larger sample of feeds is necessary to achieve more accurate predictions. The lack of influence of the correction for microbial contamination on the prediction observed in the present study increases the data available for this prediction. However, only the use of corrected values may provide an accurate evaluation. © 2017 Society of Chemical Industry.
采用氮标记技术，并考虑瘤胃颗粒粉碎率（k）和流出率（k），对10种精饲料的瘤胃未降解蛋白质（RUP）和肠道消化蛋白质（IDP）进行原位估计，估计值未校正或校正了瘤胃微生物定植情况，以检验这种校正对IDP与RUP值之间关系的影响。使用3只装有瘤胃和十二指肠瘘管的阉羊建立了这两个变量。
协方差分析表明，校正微生物定植情况并不影响这两个变量之间的紧密关系（IDP = -0.0132 ± 0.00679 + 0.776 ± 0.0002 RUP；n = 60；P < 0.001；r = 0.960）（P = 0.682）。
可根据RUP值预测精饲料和工业副产品中的IDP含量，从而避免测定肠道消化率这一繁琐复杂的过程；然而，需要更大的饲料样本才能实现更准确的预测。本研究中观察到的微生物污染校正对预测无影响，增加了可用于该预测的数据。但是，只有使用校正后的值才能提供准确的评估。© 2017化学工业协会。
