塔罗科血橙（Citrus sinensis L. Osbeck）“Lempso” 克隆品种的类黄酮成分及通过DPPH-UHPLC-PDA-IT-TOF进行的快速抗氧化活性筛选

Flavonoid Composition of Tarocco (Citrus sinensis L. Osbeck) Clone "Lempso" and Fast Antioxidant Activity Screening by DPPH-UHPLC-PDA-IT-TOF.

作者信息

Sommella Eduardo, Pagano Francesco, Pepe Giacomo, Ostacolo Carmine, Manfra Michele, Chieppa Marcello, Di Sanzo Rosa, Carabetta Sonia, Campiglia Pietro, Russo Mariateresa

机构信息

Food Chemistry Lab, Dipartimento di Agraria, Università Mediterranea di Reggio Calabria, Reggio Calabria, Via Melissari, Stecca n.4, 89122, Reggio Calabria, Italy.

Department of Pharmacy, School of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084, Fisciano, SA, Italy.

出版信息

Phytochem Anal. 2017 Nov;28(6):521-528. doi: 10.1002/pca.2701. Epub 2017 Jul 7.

DOI:10.1002/pca.2701

PMID:28686312

Abstract

INTRODUCTION

Clonal selection and hybridisation are valid strategies to obtain fruits with enhanced sensorial and nutraceutical properties. Within Citrus sinensis varieties, Tarocco clone "Lempso" is a typical product of the Calabria region (Italy) characterised by its red pulp. This is the first report concerning its accurate profiling.

OBJECTIVE

To characterise in detail the flavonoid composition of Lempso clone and to compare its antioxidant potential with other Citrus varieties by a fast screening method.

METHODOLOGY

Extracts were subjected to solid phase extraction and the qualitative/quantitative profile was elucidated through ultra-high performance liquid chromatography (UHPLC) coupled to photodiode array (PDA) and ion trap time-of-flight (IT-TOF) mass spectrometry detection, and compared to both Cleopatra mandarin (Citrus reticulata) and blood orange (Citrus sinensis (L.) Osbeck) Sanguinello varieties. The antioxidant activity was assessed by pre-column 2,2'-diphenyl-1-picrylhydrazyl (DPPH) reaction coupled to UHPLC-PDA.

RESULTS

Lempso is characterised by flavonoids (17) and anthocyanins (8). Flavanones content (Hesperidin: 57.19 ± 0.49, Vicenin-2: 4.59 ± 0.03, Narirutin: 5.78 ± 0.13 mg/100 mL) was considerably higher than Cleopatra and Sanguinello varieties. The developed DPPH-UHPLC-PDA method provides information regarding the single contributions to antioxidant activity, highlighting how Ferulic acid, Quercetin and Cyanidin derivatives possess considerable radical scavenging activity (> 50%). The total antioxidant activity was also evaluated and compared with positive controls, showing higher scavenging activity than Cleopatra and Sanguinello (IC : 333.76 ± 10.81 μg/mL vs. 452.62 ± 10.81 and 568.39 ± 26.98 μg/mL, respectively).

CONCLUSION

摘要

引言

克隆选择和杂交是获得具有增强感官和营养特性果实的有效策略。在甜橙品种中，塔罗科克隆品种“Lempso”是意大利卡拉布里亚地区的典型产品，其特点是果肉呈红色。这是关于其精确剖析的首份报告。

目的

详细表征Lempso克隆品种的类黄酮组成，并通过快速筛选方法将其抗氧化潜力与其他柑橘品种进行比较。

方法

提取物进行固相萃取，通过超高效液相色谱（UHPLC）结合光电二极管阵列（PDA）和离子阱飞行时间（IT-TOF）质谱检测来阐明定性/定量图谱，并与埃及艳后橘（Citrus reticulata）和血橙（Citrus sinensis (L.) Osbeck）桑吉内洛品种进行比较。通过柱前2,2'-二苯基-1-苦基肼（DPPH）反应结合UHPLC-PDA评估抗氧化活性。

结果

Lempso的特征在于含有17种类黄酮和8种花青素。黄烷酮含量（橙皮苷：57.19 ± 0.49，异荭草苷-2：4.59 ± 0.03，柚皮芸香苷：5.78 ± 0.13 mg/100 mL）明显高于埃及艳后橘和桑吉内洛品种。所开发的DPPH-UHPLC-PDA方法提供了关于对抗氧化活性单一贡献的信息，突出显示阿魏酸、槲皮素和花青素衍生物具有相当高的自由基清除活性（> 50%）。还评估了总抗氧化活性并与阳性对照进行比较，结果表明其清除活性高于埃及艳后橘和桑吉内洛（IC ：分别为333.76 ± 10.81 μg/mL，而埃及艳后橘和桑吉内洛分别为452.62 ± 10.81和568.39 ± 26.98 μg/mL）。