Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av. Coloso S-N, Antofagasta 1240000, Chile.
Facultad de Ciencias de la Salud, Universidad Arturo Prat, Casilla 121, Iquique 1110939, Chile.
Molecules. 2016 Feb 23;21(3):245. doi: 10.3390/molecules21030245.
Mass spectrometry has become a method of choice to characterize bioactive compounds in biological samples because of its sensitivity and selectivity. Hybrid ultra-HPLC hyphenated with Orbitrap mass analyzer is an innovative state of the art technology that allows fast and accurate metabolomic analyses. In this work the metabolites of a Chilean mistletoe endemic to the VIII region of Chile were investigated for the first time using UHPLC mass analysis (UHPLC-PDA-HESI-Orbitrap MS(n)). The anthocyanins, together with the non-pigmented phenolics were fingerprinted and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Six anthocyanins were identified, and among them, the 3-O-glycosides of delphinidin and cyanidin were the major ones. In addition, several phenolic acids (including feruloylquinic acid, feruloyl glucose, chlorogenic acid) and several flavonols (luteolin, quercetin, apigenin, isorhamnetin and glycoside derivatives) were also identified. The mistletoe leaves showed the highest antioxidant activity as measured by the DPPH radical bleaching, ferric reducing antioxidant power and superoxide anion scavenging activity tests (13.38 ± 0.47 µg/mL, 125.32 ± 5.96 µmolTE/g DW and 84.06 ± 4.59 at 100 µg/mL, respectively).
质谱分析已成为生物样品中生物活性化合物特征分析的首选方法，因为它具有高灵敏度和高选择性。与轨道阱质谱仪联用的超高效液相色谱技术是一种创新的前沿技术，可实现快速、准确的代谢组学分析。本研究首次采用超高效液相色谱-光电二极管阵列-电喷雾-轨道阱质谱联用技术（UHPLC-PDA-HESI-Orbitrap MS(n)）对智利特有的智利醉茄的代谢产物进行了研究。对花色苷和非色素酚类物质进行了指纹分析，并与通过 DPPH 自由基漂白、铁还原抗氧化能力（FRAP）、超氧阴离子清除活性测定（SA）和分光光度法测定的总酚类、类黄酮和花色苷含量所测得的抗氧化能力进行了相关性分析。共鉴定出 6 种花色苷，其中以飞燕草素和矢车菊素的 3-O-糖苷为主。此外，还鉴定出几种酚酸（包括阿魏酰奎宁酸、阿魏酰葡萄糖、绿原酸）和几种类黄酮（木樨草素、槲皮素、芹菜素、异鼠李素和糖苷衍生物）。迷迭香叶的抗氧化活性最高，DPPH 自由基清除率、铁还原抗氧化能力和超氧阴离子清除活性测定值分别为 13.38 ± 0.47 µg/mL、125.32 ± 5.96 µmolTE/g DW 和 84.06 ± 4.59（在 100 µg/mL 时）。
