γδTfh 细胞促进神经母细胞瘤中 B 细胞的成熟和抗体产生。
γδTFH cells promote B cell maturation and antibody production in neuroblastoma.
机构信息
Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.
Laboratory of Immunology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.
出版信息
BMC Immunol. 2017 Jul 7;18(1):36. doi: 10.1186/s12865-017-0216-x.
BACKGROUND
Previous studies have shown that γδ TFH cells are capable of modulating antibody production in immunized and infected mouse model. In recent studies, human γδ TFH cells are shown to contribute to the activation of humoral immunity and promote the maturation of B cells. However, little information is available on their involvement in neuroblastoma (NB) pathogenesis.
RESULTS
In the present study, the frequency of γδ TFH cells in 74 NB patients was significantly higher compared with that in 60 healthy controls. Moreover, most γδ TFH cells in NB patients had a naive phenotype with up-regulation of CD25, CD69, HLA-DR and CD40L and down-regulation of ICOS. Importantly, γδ TFH cells in NB patients produced more IL-4 and IL-10 than those in healthy controls. Furthermore, serum total IgG level was significantly increased in NB patients compared with healthy controls. The expression of CD23 on B cells was up-regulated while CD80 expression was significantly down-regulated in NB patients. Further analysis of B cell compartment showed that the frequency of CD19CD27 plasma cells was enhanced in NB patients. Spearman's correlation analysis revealed that the frequency of γδ TFH cells was positively correlated to serum total IgG level and CD19CD27 plasma cells in NB patients, but negatively correlated to CD19 B cells.
CONCLUSIONS
We concluded that γδ TFH cells might promote B cell maturation and antibody production in NB patients.
背景
先前的研究表明，γδ TFH 细胞能够调节免疫接种和感染小鼠模型中的抗体产生。在最近的研究中，人类 γδ TFH 细胞被证明有助于激活体液免疫并促进 B 细胞成熟。然而，关于它们在神经母细胞瘤 (NB) 发病机制中的参与情况的信息很少。
结果
在本研究中，与 60 名健康对照者相比，74 名 NB 患者中 γδ TFH 细胞的频率明显更高。此外，NB 患者中的大多数 γδ TFH 细胞具有幼稚表型，表现为 CD25、CD69、HLA-DR 和 CD40L 上调，ICOS 下调。重要的是，NB 患者中的 γδ TFH 细胞比健康对照组产生更多的 IL-4 和 IL-10。此外，与健康对照组相比，NB 患者的血清总 IgG 水平显著升高。NB 患者的 B 细胞上 CD23 的表达上调，而 CD80 的表达显著下调。对 B 细胞区室的进一步分析显示，NB 患者中 CD19CD27 浆细胞的频率增加。Spearman 相关分析表明，NB 患者中 γδ TFH 细胞的频率与血清总 IgG 水平和 CD19CD27 浆细胞呈正相关，与 CD19 B 细胞呈负相关。
结论
我们得出结论，γδ TFH 细胞可能促进 NB 患者的 B 细胞成熟和抗体产生。
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