Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università degli Studi di Palermo, Palermo, Italy.
PLoS One. 2012;7(7):e41940. doi: 10.1371/journal.pone.0041940. Epub 2012 Jul 25.
Vγ9Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and display pleiotropic features. Here we report that coculture of Vγ9Vδ2 cells with phosphoantigen and IL-21 leads to selective expression of the transcription repressor Bcl-6 and polarization toward a lymphocyte subset displaying features of follicular B-helper T (T(FH)) cells. T(FH)-like Vγ9Vδ2 cells have a predominant central memory (CD27(+)CD45RA(-)) phenotype and express ICOS, CD40L and CXCR5. Upon antigen activation, they secrete IL-4, IL-10 and CXCL13, and provide B-cell help for antibody production in vitro. Our findings delineate a subset of human Vγ9Vδ2 lymphocytes, which, upon interaction with IL-21-producing CD4 T(FH) cells and B cells in secondary lymphoid organs, is implicated in the production of high affinity antibodies against microbial pathogens.
Vγ9Vδ2 T 淋巴细胞在 MHC 分子呈递非肽抗原的情况下识别，并表现出多效性特征。在这里，我们报告说，Vγ9Vδ2 细胞与磷酸抗原和 IL-21 共培养会导致转录抑制因子 Bcl-6 的选择性表达，并向具有滤泡 B 辅助 T（T(FH)）细胞特征的淋巴细胞亚群极化。T(FH)样 Vγ9Vδ2 细胞具有主要的中央记忆（CD27(+)CD45RA(-)）表型，并表达 ICOS、CD40L 和 CXCR5。在抗原激活后，它们分泌 IL-4、IL-10 和 CXCL13，并在体外为 B 细胞产生抗体提供 B 细胞帮助。我们的研究结果描绘了人类 Vγ9Vδ2 淋巴细胞的一个亚群，该亚群在与二级淋巴器官中产生 IL-21 的 CD4 T(FH)细胞和 B 细胞相互作用时，参与了针对微生物病原体的高亲和力抗体的产生。
