Department of Polymer Science, The University of Akron, Akron, OH 44325, United States.
Department of Polymer Science, The University of Akron, Akron, OH 44325, United States; Department of Biomedical Engineering, The University of Akron, Akron, OH 44325, United States.
Biomaterials. 2017 Oct;141:176-187. doi: 10.1016/j.biomaterials.2017.06.038. Epub 2017 Jun 28.
Additive manufacturing has the potential to revolutionize regenerative medicine, but the harsh thermal or photochemical conditions during the 3D printing process limit the inclusion of drugs, growth factors and other biologics within the resulting scaffolds. Functionalization strategies that enable specific placement of bioactive species on the surface of 3D printed structures following the printing process afford a promising approach to sidestep the harsh conditions and incorporate these valuable bioactive molecules with precise control over concentration. Herein, resorbable polymer scaffolds were prepared from propargyl functionalized L-phenylalanine-based poly(ester urea)s (PEUs). Osteogenic growth peptide (OGP) or bone morphogenic protein-2 (BMP-2) peptides were immobilized on PEU scaffolds through surface available propargyl groups via copper-catalyzed azide alkyne cycloaddition (CuAAC) post 3D printing. The presence of either OGP or BMP-2 significantly enhanced hMSCs osteogenic differentiation compared to unfunctionalized scaffolds.
增材制造有可能彻底改变再生医学，但在 3D 打印过程中的苛刻热或光化学条件限制了药物、生长因子和其他生物制剂在所得支架内的包含。在打印过程之后能够在 3D 打印结构的表面上进行特定的生物活性物质放置的功能化策略提供了一种有前途的方法来规避苛刻的条件，并将这些有价值的生物活性分子与对浓度的精确控制结合起来。在此，通过铜催化的叠氮-炔环加成（CuAAC）后 3D 打印，从炔丙基功能化的基于 L-苯丙氨酸的聚（酯脲）（PEU）制备了可吸收聚合物支架。成骨生长肽（OGP）或骨形态发生蛋白-2（BMP-2）肽通过表面可用的炔丙基基团固定在 PEU 支架上。与未功能化的支架相比，存在 OGP 或 BMP-2 显著增强了 hMSCs 的成骨分化。
