van der Meulen Talitha, Swarts Spencer, Fischer Wolfgang, van der Geer Peter
Department of Chemistry and Biochemistry, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-1030, USA.
Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, 10010 North Torrey Pines Rd, La Jolla, CA 92037, USA.
Biochem Biophys Res Commun. 2017 Sep 2;490(4):1334-1339. doi: 10.1016/j.bbrc.2017.07.024. Epub 2017 Jul 6.
ShcA is a cytoplasmic signaling protein that supports signal transduction by receptor protein-tyrosine kinases by providing auxiliary tyrosine phosphorylation sites that engage additional signaling proteins. The principal binding partner for tyrosine phosphorylation sites on ShcA is Grb2. In the current study, we have used phosphotyrosine-containing peptides to isolate and identify STS-1 as a novel ShcA-binding protein. Our results further show that the interaction between STS-1 and ShcA is regulated in response to EGF receptor activation.
ShcA是一种细胞质信号蛋白，它通过提供与其他信号蛋白结合的辅助酪氨酸磷酸化位点，来支持受体蛋白酪氨酸激酶的信号转导。ShcA上酪氨酸磷酸化位点的主要结合伴侣是Grb2。在本研究中，我们使用含磷酸酪氨酸的肽段来分离并鉴定STS-1为一种新型的ShcA结合蛋白。我们的结果进一步表明，STS-1与ShcA之间的相互作用受表皮生长因子受体激活的调控。
