聚多巴胺固定化聚丙烯微量离心管作为一种pH响应平台用于从食源性病原体中捕获/释放DNA
Polydopamine-immobilized polypropylene microfuge tube as a pH-responsive platform for capture/release of DNA from foodborne pathogens.
作者信息
Zhong Zitao, Yao Xin, Gao Xiaomei, Jia Li
机构信息
Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
出版信息
Anal Biochem. 2017 Oct 1;534:14-18. doi: 10.1016/j.ab.2017.07.004. Epub 2017 Jul 6.
A rapid, convenient and efficient DNA extraction method with no need for toxic agents and centrifugation was reported. A polypropylene microfuge (MF) tube was used as the substrate to immobilize polydopamine (PDA). The prepared PDA-coated MF (PDA@MF) tube was used as a pH-responsive platform for rapid extraction of DNA based on pH-induced charge switch of amino and phenolic hydroxyl groups in PDA coating. The extraction procedure is simple and can be finished in 25 min. The PDA@MF tube was applied for extraction of genomic DNA from foodborne pathogens in milk. The extracted DNA was directly used as template for PCR amplification.
报道了一种快速、便捷且高效的DNA提取方法，该方法无需使用有毒试剂且无需离心。使用聚丙烯微量离心（MF）管作为固定聚多巴胺（PDA）的基质。制备的涂覆有PDA的MF（PDA@MF）管用作基于PDA涂层中氨基和酚羟基的pH诱导电荷转换的快速DNA提取的pH响应平台。提取过程简单，可在25分钟内完成。PDA@MF管用于从牛奶中的食源性病原体提取基因组DNA。提取的DNA直接用作PCR扩增的模板。
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